Effect of the Ionizing Radiation Dose on Histone H2AX

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23220%2F21%3A43963762" target="_blank" >RIV/49777513:23220/21:43963762 - isvavai.cz</a>

Alternative codes found

RIV/49777513:23310/21:43963762

Result on the web

<a href="https://www.djs.si/nene2021/proceedings/pdf/NENE2021_1015.pdf" target="_blank" >https://www.djs.si/nene2021/proceedings/pdf/NENE2021_1015.pdf</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Effect of the Ionizing Radiation Dose on Histone H2AX

Original language description

In case the radiation dosimeters are not available, biological dosimetry represents an important method to estimate the absorbed dose of the exposed individuals during nuclear events. Nevertheless, the eukaryotic DNA is constantly exposed to exogenous and endogenous factors. Apart from the ionizing radiation, widescale DNA lesions are also induced by other harmful effects. DNA double-strand breaks (DSBs) are the gravest lesions. DSBs provoke an extensive reaction characterized by the expression of the H2AX molecule. The scope of this work is an assessment of a gamma radiation dose-effect on a human body in terms of expression of the H2AX in DNA. This contribution is focused on the expression scale of the phosphorylated H2AX molecule (g-H2AX), which highlights a DNA damage induced by the exposure to gamma radiation. The dependency between the share of g-H2AX molecule in an irradiated sample and the radiation dose was examined. The investigated subjects consist of fourteen samples of uncoagulable blood from healthy donors. The sample of each donor was divided into four test tubes – a negative control + three levels of gamma radiation (0.5 Gray, 1 Gray, 2 Gray). The irradiation was performed on a medical caesium source “Gammacell® 1000 Elite.” The evaluation was based on the method for determining g-H2AX after chemical stimulation DNA – extracorporeal photopheresis. The outcome of this work is the confirmation that the production of this molecule is dependent on the dose of gamma radiation. Owing to the g-H2AX characteristic, the finding of the relation between the share of g-H2AX molecule in a sample and a dose of radiation was statistically confirmed. Accordingly, the H2AX molecule can be considered a reliable specific marker for DNA damage. In the future, this method could find a purpose in practical events, for example, re-determination dose of radiation after nuclear events.

Czech name

—

Czech description

—

Type

D - Article in proceedings

CEP classification

—

OECD FORD branch

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the International Conference Nuclear Energy for New Europe (NENE 2021)

ISBN

978-961-6207-51-5

ISSN

—

e-ISSN

—

Number of pages

7

Pages from-to

"1015.1"-"1015.7"

Publisher name

Nuclear Society of Slovenia

Place of publication

Ljubljana

Event location

on-line, Bled, Slovenia

Event date

Sep 6, 2021

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

—