Novel morphological multi-scale evaluation system for quality assessment of decellularized liver scaffolds

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F49777513%3A23520%2F20%3A43959830" target="_blank" >RIV/49777513:23520/20:43959830 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1177/2041731420921121" target="_blank" >https://doi.org/10.1177/2041731420921121</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1177/2041731420921121" target="_blank" >10.1177/2041731420921121</a>

Result language

angličtina

Original language name

Novel morphological multi-scale evaluation system for quality assessment of decellularized liver scaffolds

Original language description

Decellularized scaffolds can serve as an excellent three-dimensional environment for cell repopulation. They maintain tissue-specific microarchitecture of extracellular matrix proteins with important spatial cues for cell adhesion, migration, growth, and differentiation. However, criteria for quality assessment of the three-dimensional structure of decellularized scaffolds are rather fragmented, usually study-specific, and mostly semi-quantitative. Thus, we aimed to develop a robust structural assessment system for decellularized porcine liver scaffolds. Five scaffolds of different quality were used to establish the new evaluation system. We combined conventional semi-quantitative scoring criteria with a quantitative scaffold evaluation based on automated image analysis. For the quantitation, we developed a specific open source software tool (ScaffAn) applying algorithms designed for texture analysis, segmentation, and skeletonization. ScaffAn calculates selected parameters characterizing structural features of porcine liver scaffolds such as the sinusoidal network. After evaluating individual scaffolds, the total scores predicted scaffold interaction with cells in terms of cell adhesion. Higher scores corresponded to higher numbers of cells attached to the scaffolds. Moreover, our analysis revealed that the conventional system could not identify fine differences between good quality scaffolds while the additional use of ScaffAn allowed discrimination. This led us to the conclusion that only using the combined score resulted in the best discrimination between different quality scaffolds. Overall, our newly defined evaluation system has the potential to select the liver scaffolds most suitable for recellularization, and can represent a step toward better success in liver tissue engineering.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30402 - Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction)

Project

<a href="/en/project/EF17_048%2F0007280" target="_blank" >EF17_048/0007280: Application of Modern Technologies in Medicine and Industry</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Tissue Engineering

ISSN

1932-6254

e-ISSN

—

Volume of the periodical

11

Issue of the periodical within the volume

May

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

—

UT code for WoS article

000538875600001

EID of the result in the Scopus database

2-s2.0-85085612560