Molecular genetic analysis of the locus of complex vertebral malformation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F06%3A00007165" target="_blank" >RIV/60076658:12220/06:00007165 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Molekulárně genetická analýzy lokusu pro komplex vertebrálních malformací

Original language description

The polymorphism in the gene of CVM was analysed. The heterozygous carriers (CV) and dominant homozygotes (TV) were genotyped. DNA was isolated from the whole blood of elite Holstein females, mothers of sires or embryo donors. The gene was amplified by parts in polymerase chain reaction, the restriction fragment length polymorphism analyse was done (PCR/RFLP). The polymorphic restriction site of AvaII, HaeIII, AvaI and AvaII was found, but the mutation was not linked to the recessive allele of CVM, as polymorphic variants occurred in CV and TV animals.

Czech name

Molekulárně genetická analýzy lokusu pro komplex vertebrálních malformací

Czech description

The polymorphism in the gene of CVM was analysed. The heterozygous carriers (CV) and dominant homozygotes (TV) were genotyped. DNA was isolated from the whole blood of elite Holstein females, mothers of sires or embryo donors. The gene was amplified by parts in polymerase chain reaction, the restriction fragment length polymorphism analyse was done (PCR/RFLP). The polymorphic restriction site of AvaII, HaeIII, AvaI and AvaII was found, but the mutation was not linked to the recessive allele of CVM, as polymorphic variants occurred in CV and TV animals.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Collection of Scientific Papers, Faculty of Agriculture in České Budějovice : Series for Animal Sciences

ISSN

1212-558X

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

39-42

UT code for WoS article

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EID of the result in the Scopus database

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