Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F10%3A00012493" target="_blank" >RIV/60076658:12220/10:00012493 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Validation of microarray analysis and identification of differentially expressed genes in two durum wheat cultivars with contrasting response to drought stress

Original language description

The durum wheat cultivars ?Cappelli? and ?Ofanto? are characterized by a clear differences in response to heat and drought stress. Wheat Affymetrix microarray was used in the two cultivars subjected to water stress at booting stage. Differential expressed genes with different stress expression profiles were found in Cappelli vs. Ofanto. According to the fold change threshold of the Affymetrix dataset and the annotated activity among these genes drought response gene markers were selected and some of them have been validated by qRT-PCR for their stabile behaving during plant development in an experiment made with two cultivars under drought stress at tillering developmental stages. These results will open the possibility to identify expression QTLs controlling the response of durum wheat to abiotic stress making possible the understanding of the genetic bases of stress dependent gene expression.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GD521%2F08%2FH042" target="_blank" >GD521/08/H042: Molecular biology, ecology and taxonomy</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Úroda : časopis pro rostlinnou produkci

ISSN

0139-6013

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

12

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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