THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F17%3A43896699" target="_blank" >RIV/60076658:12220/17:43896699 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.5593/sgem2017/52/S20.126" target="_blank" >http://dx.doi.org/10.5593/sgem2017/52/S20.126</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.5593/sgem2017/52/S20.126" target="_blank" >10.5593/sgem2017/52/S20.126</a>
Alternative languages
Result language
angličtina
Original language name
THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)
Original language description
Pectinatella magnifica is freshwater organism, which round-shaped colonies are most often found attached to the different submerged parts of coastal plants and can reach the weight even several kilograms. Usually it can be found in oligotrophic to mesotrophic waters, during the season when water temperature reaches 20°C and more [4]. Although Pectinatella magnifica originally comes from North America, it has recently been known as an invasive species in Europe and part of Asia. The first recorded occurrence in Europe was in 1983 near Hamburg [2] in the Czech Republic it was recorded for the first time in rivers Vltava and Labe in the first half of the 20th century [9]. The most common way of its propagation is through internal buds, called statoblasts, which are fitted with hooks. These anchor-shaped hooks make statoblasts able to catch to any surface, and allow easy spread over long distances and different terrain. The aim of this study was to compare different methods of isolation of DNA from colonies of Pectinatella magnifica. Three main different techniques of DNA extraction from zooids and statoblasts were tested, namely two kit-based methods: DNeasy Plant Mini isolation kit (QIAGEN), Nucleo Spin isolation kit (Marcherey-Nagel) and one isolation method based on cetyltrimethylammonium bromide (CTAB-PVP). There were also tested differences between statoblast and zooid DNA and between natural stored and ethanol fixed samples. Material for analysis was collected at the Protected Landscape Area (PLA) and a Biosphere Reserve (BR) of Třeboňsko
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10603 - Genetics and heredity (medical genetics to be 3)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Int. Multidisciplinary Sci.Geoconf. Surveying Geology Mining Ecology Manage.,SGEM
ISBN
978-619-7408-09-6
ISSN
1314-2704
e-ISSN
neuvedeno
Number of pages
8
Pages from-to
989-996
Publisher name
International Multidisciplinary Scientific Geoconference
Place of publication
Albena
Event location
Albena
Event date
Jun 29, 2017
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
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