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THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F17%3A43896699" target="_blank" >RIV/60076658:12220/17:43896699 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.5593/sgem2017/52/S20.126" target="_blank" >http://dx.doi.org/10.5593/sgem2017/52/S20.126</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.5593/sgem2017/52/S20.126" target="_blank" >10.5593/sgem2017/52/S20.126</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    THE EXTRACTION OF DNA FROM COLONIES OF PECTINATELLA MAGNIFICA (LEIDY 1851)

  • Original language description

    Pectinatella magnifica is freshwater organism, which round-shaped colonies are most often found attached to the different submerged parts of coastal plants and can reach the weight even several kilograms. Usually it can be found in oligotrophic to mesotrophic waters, during the season when water temperature reaches 20°C and more [4]. Although Pectinatella magnifica originally comes from North America, it has recently been known as an invasive species in Europe and part of Asia. The first recorded occurrence in Europe was in 1983 near Hamburg [2] in the Czech Republic it was recorded for the first time in rivers Vltava and Labe in the first half of the 20th century [9]. The most common way of its propagation is through internal buds, called statoblasts, which are fitted with hooks. These anchor-shaped hooks make statoblasts able to catch to any surface, and allow easy spread over long distances and different terrain. The aim of this study was to compare different methods of isolation of DNA from colonies of Pectinatella magnifica. Three main different techniques of DNA extraction from zooids and statoblasts were tested, namely two kit-based methods: DNeasy Plant Mini isolation kit (QIAGEN), Nucleo Spin isolation kit (Marcherey-Nagel) and one isolation method based on cetyltrimethylammonium bromide (CTAB-PVP). There were also tested differences between statoblast and zooid DNA and between natural stored and ethanol fixed samples. Material for analysis was collected at the Protected Landscape Area (PLA) and a Biosphere Reserve (BR) of Třeboňsko

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

  • OECD FORD branch

    10603 - Genetics and heredity (medical genetics to be 3)

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Int. Multidisciplinary Sci.Geoconf. Surveying Geology Mining Ecology Manage.,SGEM

  • ISBN

    978-619-7408-09-6

  • ISSN

    1314-2704

  • e-ISSN

    neuvedeno

  • Number of pages

    8

  • Pages from-to

    989-996

  • Publisher name

    International Multidisciplinary Scientific Geoconference

  • Place of publication

    Albena

  • Event location

    Albena

  • Event date

    Jun 29, 2017

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article