Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F18%3A43897690" target="_blank" >RIV/60076658:12220/18:43897690 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14740/18:00106168
Result on the web
<a href="http://dx.doi.org/10.1007/s00253-018-9373-y" target="_blank" >http://dx.doi.org/10.1007/s00253-018-9373-y</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00253-018-9373-y" target="_blank" >10.1007/s00253-018-9373-y</a>
Alternative languages
Result language
angličtina
Original language name
Proteomic characterization and antifungal activity of potato tuber proteins isolated from starch production waste under different temperature regimes
Original language description
Proteins were obtained from effluent of a starch manufacture by using different isolation temperatures (40, 60, 80, and 100 °C). The proteins, remaining in effluent after treatment of potato juice at 80 and 100 °C differed significantly in composition and in structural stability as well as in trypsin inhibitory and antifungal activities in comparison with the variants of 40 and 60 °C. The protein samples of 80 °C exhibited the highest antifungal activity and its average value of IC50 against five strains of two Fusarium species was determined in average at 0.18 mg ml−1. The 80 °C protein samples consisted predominantly of lowmolecular proteins (7–17 kDa) identified as potato tuber protease inhibitors I and II. Predominantly, protease inhibitors II were identified for the protein samples obtained by 100 °C and here we identified 7 spots in comparison with 12 identified for the 80 °C samples. Samples of 40 and 60 °C with low antifungal activities represent high variability of detected and identified proteins.We identified various representatives of aspartic, cysteine, and serine protease inhibitors in both types of samples. These samples also contained Kunitz-type protease inhibitors that were not found in the 80 and 100 °C samples which documented thermal unstableness of Kunitz-type protease inhibitors. Functional stability at high temperatures and antifungal activity of isolated potato protease inhibitors I and II support the potential of this fraction usage in food, feed, pharmaceutical, or agricultural industry and offer new products for starch manufactures. At the same time, utilization of the stable protein fraction of waste deproteinized potato water promotes exploitation of potato starch production resources.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
<a href="/en/project/LM2015043" target="_blank" >LM2015043: Czech Infrastructure for Integrative Structural Biology</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Applied Microbiology and Biotechnology
ISSN
0175-7598
e-ISSN
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Volume of the periodical
102
Issue of the periodical within the volume
24
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
10551-10560
UT code for WoS article
000451523800017
EID of the result in the Scopus database
2-s2.0-85054294321