Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F18%3A43897743" target="_blank" >RIV/60076658:12220/18:43897743 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2754/avb201887010019" target="_blank" >http://dx.doi.org/10.2754/avb201887010019</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb201887010019" target="_blank" >10.2754/avb201887010019</a>

Result language
angličtina
Original language name
Genetic characterisation of small ruminant lentiviruses in sheep and goats from the Czech Republic
Original language description
The aim of this study was to determine the prevalence of small ruminant lentivirus (SRLV) infections on sheep and goat farms which are exempt from state monitoring and carry molecular characterisation of strains circulating amongst these farms without SRLV eradication. A total number of 3,410 blood samples of sheep and goats from 21 herds were collected for the purpose of the project. The detected serological prevalence of maedi visna in sheep was 19.9% (556/2801) and the seroprevalence of caprine arthritis and encephalitis in goats was 14.1% (86/609). All positive animals were tested by the nested polymerase chain reaction (nPCR) method for the presence of provirus in the buffy-coats from EDTA-blood samples. Phylogenetic analysis of 93 SRLV strains identified the genotype in 77 sequences, where 60 of them were genotype A and 17 belonged to genotype B. Whereas all of the genotype B sequences were classified in subtype B2, the genotype A group of isolates showed higher variability and were related to subgenotypes A2 and A3. This study represents the first report of genetic characterisation of SRLV strains circulating in the territory of the Czech Republic.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40301 - Veterinary science

Project
<a href="/en/project/QJ1610096" target="_blank" >QJ1610096: The program of lentiviral infection health check in small ruminants using new methods of early laboratory diagnostics and markers of genetically determined resistance to infection as a selection criterion</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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