Cryptosporidium ratti n. sp. (Apicomplexa: Cryptosporidiidae) and genetic diversity of Cryptosporidium spp. in brown rats (Rattus norvegicus) in the Czech Republic
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F21%3A43903271" target="_blank" >RIV/60076658:12220/21:43903271 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/21:00554115
Result on the web
<a href="https://www.cambridge.org/core/journals/parasitology/article/abs/cryptosporidium-ratti-n-sp-apicomplexa-cryptosporidiidae-and-genetic-diversity-of-cryptosporidium-spp-in-brown-rats-rattus-norvegicus-in-the-czech-republic/1D7044BF78B1B70A8E7FAE48FD996710" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/abs/cryptosporidium-ratti-n-sp-apicomplexa-cryptosporidiidae-and-genetic-diversity-of-cryptosporidium-spp-in-brown-rats-rattus-norvegicus-in-the-czech-republic/1D7044BF78B1B70A8E7FAE48FD996710</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0031182020001833" target="_blank" >10.1017/S0031182020001833</a>
Alternative languages
Result language
angličtina
Original language name
Cryptosporidium ratti n. sp. (Apicomplexa: Cryptosporidiidae) and genetic diversity of Cryptosporidium spp. in brown rats (Rattus norvegicus) in the Czech Republic
Original language description
The diversity and biology of Cryptosporidium that is specific for rats (Rattus spp.) are not well studied. We examined the occurrence and genetic diversity of Cryptosporidium spp. in wild brown rats (Rattus norvegicus) by microscopy and polymerase chain reaction (PCR)/sequencing targeting the small subunit rDNA (SSU), actin and HSP70 genes. Out of 343 faecal samples tested, none were positive by microscopy and 55 were positive by PCR. Sequence analysis of SSU gene revealed the presence of Cryptosporidium muris (n = 4), C. andersoni (n = 3), C. ryanae (n = 1), C. occultus (n = 3), Cryptosporidium rat genotype I (n = 23), Cryptosporidium rat genotype IV (n = 16) and novel Cryptosporidium rat genotype V (n = 5). Spherical oocysts of Cryptosporidium rat genotype I obtained from naturally-infected rats, measuring 4.4-5.4 mu m x 4.3-5.1 mu m, were infectious to the laboratory rats, but not to the BALB/c mice (Mus musculus) nor Mongolian gerbils (Meriones unguiculatus). The prepatent period was 3 days post infection and the patent period was longer than 30 days. Naturally- and experimentally-infected rats showed no clinical signs of disease. Percentage of nucleotide similarities at the SSU, actin, HSP70 loci between C. ratti n. sp. and the rat derived C. occultus and Cryptosporidium rat genotype II, III, IV, and V ranged from 91.0 to 98.1%. These genetic variations were similar or greater than that observed between closely related species, i.e. C. parvum and C. erinacei (93.2-99.5%). Our morphological, genetic and biological data support the establishment of Cryptosporidium rat genotype I as a new species, Cryptosporidium ratti n. sp.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10613 - Zoology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasitology
ISSN
0031-1820
e-ISSN
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Volume of the periodical
148
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
84-97
UT code for WoS article
000607400500007
EID of the result in the Scopus database
2-s2.0-85092180149