Development of RT-PCR for rapid detection of ssRNA ambi-like mycovirus in a root rot fungi (Armillaria spp.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F22%3A43905896" target="_blank" >RIV/60076658:12220/22:43905896 - isvavai.cz</a>
Result on the web
<a href="http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7828&category_id=184&option=com_virtuemart" target="_blank" >http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7828&category_id=184&option=com_virtuemart</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4149/av_2022_308" target="_blank" >10.4149/av_2022_308</a>

Result language
angličtina
Original language name
Development of RT-PCR for rapid detection of ssRNA ambi-like mycovirus in a root rot fungi (Armillaria spp.)
Original language description
Plant pathogenic fungi cause severe losses and damages to crops worldwide. Fungal antagonists including viruses can play a significant role in controlling of these phytopathogens and reduce disease risk in plants. Understanding the mode of action of mycoviruses is essential to achieve optimum host control. Although the number of described mycoviruses is increasing due to the ability to detect different mycoviruses using transcriptome approaches, there is a need to identify and isolate new viruses from phytopathogenic fungi that can introduce new possibilities to control serious plants pests. The rapid, reliable and cost-effective screening of fungi is required when searching for new potentially effective biological control products. Here, we evaluated the efficacy of the reverse transcription PCR (RT-PCR) for fast detection using samples from Armillaria sp. mycovirus survey. After testing 12 strains of Armillaria spp., 2 were found to be positive for ambisense viruses by RT-PCR. Ambisense ssRNA viruses was detectable in all symptomless mycelia, including strains maintained in fungal culture collection for decades.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/QK1920412" target="_blank" >QK1920412: Mycoviruses as potential agents in the protection of spruce stands against honey fungus</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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