Selection of reference genes for expression profiling in biostimulation research of soybean
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F24%3A43908344" target="_blank" >RIV/60076658:12220/24:43908344 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12410/24:43908344
Result on the web
<a href="https://chembioagro.springeropen.com/articles/10.1186/s40538-024-00660-3" target="_blank" >https://chembioagro.springeropen.com/articles/10.1186/s40538-024-00660-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s40538-024-00660-3" target="_blank" >10.1186/s40538-024-00660-3</a>
Alternative languages
Result language
angličtina
Original language name
Selection of reference genes for expression profiling in biostimulation research of soybean
Original language description
BackgroundPlant biostimulants constitute a promising environmentally friendly alternative for increasing crop yield and tolerance to unfavorable conditions. Among various types of such formulations, botanical extracts are gaining more recognition as products supporting plant performance. Moreover, novel tools such as cold-plasma or low-pressure microwave plasma discharge are being proposed as techniques that might improve their efficacy. Elucidation of the biostimulant's mode of action requires complex research at a molecular level. Transcriptional changes occurring after biostimulant spraying might be investigated using RT-qPCR. However, this technique requires data normalization against stable endogenous controls.ResultsHere, we tested the expression stability of ten candidate genes in soybean plants exposed to various biostimulants treatment. Selection of the best-performing reference genes was conducted using four algorithms (geNorm, NormFinder, BestKeeper, and Delta Ct method). According to the obtained results, Bic-C2 (RNA-binding protein Bicaudal-C) and CYP (cyclophilin type peptidyl-prolyl cis-trans isomerase) showed highest expression stability, while expression of EF1B (elongation factor 1-beta) fluctuated the most among a tested set of candidate genes.ConclusionsOverall, we recommend using Bic-C2 together with CYP for the RT-qPCR data normalization in soybean biostimulation experiments. To our best knowledge, this is the first comprehensive study of reference genes stability in plants subjected to biostimulant treatment. The results of this study will aid in further biostimulant research in crop plants, facilitating analyses performed on the transcriptional level.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
40106 - Agronomy, plant breeding and plant protection; (Agricultural biotechnology to be 4.4)
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chemical and Biological Technologies in Agriculture
ISSN
2196-5641
e-ISSN
2196-5641
Volume of the periodical
11
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
"1–12"
UT code for WoS article
001304011200001
EID of the result in the Scopus database
2-s2.0-85203026165