Understanding the molecular mechanisms of interactions between biochar and denitrifiers in N₂O emissions reduction: Pathway to more economical and sustainable fertilizers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F25%3A43908547" target="_blank" >RIV/60076658:12220/25:43908547 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12510/25:43908547
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0167198724004069" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0167198724004069</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.still.2024.106405" target="_blank" >10.1016/j.still.2024.106405</a>
Alternative languages
Result language
angličtina
Original language name
Understanding the molecular mechanisms of interactions between biochar and denitrifiers in N₂O emissions reduction: Pathway to more economical and sustainable fertilizers
Original language description
Biochar application to topsoil has been repeatedly and independently reported to reduce N2O emissions, yet the underlying mechanisms remain poorly understood. This study hypothesizes that biochar enhances the stability and catalytic activity of N2O reductase enzymes in denitrifying bacteria, promoting the conversion of N2O to N2 during denitrification. Interactions between biochar and the N2O reductase enzyme (PsN2OR) from the denitrifying bacterium Pseudomonas stutzeri were investigated through molecular dynamics simulations. The obtained results firstly revealed that biochar stabilizes this periplasmic enzyme in the aqueous solution via hydrophobic and hydrophilic interactions. Specifically, π–π stacking and hydrophobic interactions reduce the thermal fluctuations of hydrophobic amino acids, lowering entropy and improving enzymatic efficiency. Additionally, biochar adsorbs N2O molecules, facilitating their delivery to the active site of the enzyme and enhancing the reaction rate. Deeper understandings of molecular interactions open new pathways in developing biochar-based fertilizers with slower, more economically and more environmentally favorable release of nutrients. This new type of fertilizers creates new opportunities for the biochar market, positioning it as a valuable tool for carbon sequestration and the mitigation of N₂O emissions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
50202 - Applied Economics, Econometrics
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Soil and Tillage Research
ISSN
0167-1987
e-ISSN
1879-3444
Volume of the periodical
248
Issue of the periodical within the volume
2025
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
1-6
UT code for WoS article
001382308800001
EID of the result in the Scopus database
2-s2.0-85211069101