Development of an algae extract-based culture medium for Paenibacillus larvae without animal-derived components

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12220%2F25%3A43910226" target="_blank" >RIV/60076658:12220/25:43910226 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S2215016125004820?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2215016125004820?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mex.2025.103638" target="_blank" >10.1016/j.mex.2025.103638</a>

Result language

angličtina

Original language name

Development of an algae extract-based culture medium for Paenibacillus larvae without animal-derived components

Original language description

The cultivation of Paenibacillus larvae, the etiological agent of American foulbrood, traditionally relies on media containing animal-derived nutrients. In response to ethical and practical concerns associated with such components, we developed and validated a new culture medium based on an algae extract. The formulation includes Chlorella as the principal nutrient source and is supplemented with uric acid and L-tyrosine to support spore germination under conditions resembling the honeybee larval gut. The method includes optimized procedures for dissolving sparingly soluble components and minimizing nutrient degradation. Performance of the new medium was comparable to that of MYPGP and other conventional media in supporting spore germination and vegetative growth of P. larvae.• Eliminates the need for animal-derived components through the use of microalgal nutrients• Supports robust germination and colony development of P. Larvae• Provides an ethical and cost-efficient medium suitable for diagnostic and research applications

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MethodsX

ISSN

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e-ISSN

2215-0161

Volume of the periodical

15

Issue of the periodical within the volume

103638

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

1-5

UT code for WoS article

001584893800002

EID of the result in the Scopus database

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