Structurally unique recombinant Kazal-type proteinase inhibitor retains activity when terminally extended and glycosylated

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F05%3A00008707" target="_blank" >RIV/60076658:12310/05:00008707 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Structurally unique recombinant Kazal-type proteinase inhibitor retains activity when terminally extended and glycosylated

Original language description

Recombinant derivatives of the Kazal-type serine proteinase inhibitor GmSP12 (36 amino acid residues), which is a component of insect silk, were prepared in the expression vector Pichia pastoris. The rhSP12 had a C-terminal hexahistidine tag attached tothe GmSP12 sequence, rtSP12 was extended with GluAlaAla at the N-terminus, and rfSP12 included this N-terminal extension and a C-terminal tail of 22 residues (myc epitope and hexahistidine). A portion of the secreted rfS12 was O-glycosylated with a trimannosyl or hexamannosyl. The native inhibitor was active slightly on trypsin and highly on subtilisin and protemase K. The extended C-terminus in rhSP12 and rfSP12 enhanced activity on the two latter enzymes and rendered rfSP12 active on clastase and pronase, but abolished the inhibition of trypsin. The glycosylation of rfSP12 reduced its inhibitory activity to a level comparable with the native inhibitor. The rtSP12 with tripeptide extension at the N-terminus and no C-terminal modificati

Czech name

Strukturně unikátní inhibitor rekombinantní proteázy typu Kazal udrží aktivitu, když je na konci prodloužen a glykosylován

Czech description

Strukturně unikátní inhibitor rekombinantní proteázy typu Kazal udrží aktivitu, když je na konci prodloužen a glykosylován

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

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Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Protein expression and purification

ISSN

1046-5928

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

94-102

UT code for WoS article

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EID of the result in the Scopus database

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