Structural study of elements of Tetrahymena telomerase RNA stem-loop IV domain important for function
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F06%3A00008791" target="_blank" >RIV/60076658:12310/06:00008791 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Structural study of elements of Tetrahymena telomerase RNA stem-loop IV domain important for function
Original language description
Tetrahymena telomerase RNA (TER) contains several regions in addition to the template that are important for function. Central among these is the stem-loop IV domain, which is involved in both catalysis and RNP assembly, and includes binding sites for both the holoenzyme assembly protein p65 and telomerase reverse transcriptase (TERT). Stem-loop IV contains two regions with high evolutionary sequence conservation: a central GA bulge between helices, and a terminal loop. We solved the solution structureof loop IV and modeled the structure of the helical region containing the GA bulge, using NMR and residual dipolar couplings. The central GA bulge with flanking C-G base pairs induces a 50° semi-rigid bend in the helix. Loop IV is highly structured, andcontains a conserved C-U base pair at the top of the helical stem. Analysis of new and previous biochemical data in light of the structure provides a rationale for some of the sequence conservation in this region of TER. The results sugge
Czech name
Studium elementů struktury vlásenky IV telomerázové RNA z Tetrahymeny důležitých pro funkci.
Czech description
Studium elementů struktury vlásenky IV telomerázové RNA z Tetrahymeny důležitých pro funkci.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
RNA-A Publication of the RNA Society
ISSN
1355-8382
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
1475-1485
UT code for WoS article
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EID of the result in the Scopus database
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