Structural model of lymphocyte receptor NKR-P1C revealed bymass spectrometry and molecular modelling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F13%3A43883864" target="_blank" >RIV/60076658:12310/13:43883864 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1021/ac302860m" target="_blank" >http://dx.doi.org/10.1021/ac302860m</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/ac302860m" target="_blank" >10.1021/ac302860m</a>

Result language

angličtina

Original language name

Structural model of lymphocyte receptor NKR-P1C revealed bymass spectrometry and molecular modelling

Original language description

NKR-P1C is an activating immune receptor expressed on the surface of mouse natural killer cells. It has been widely used as a marker for NK cell identification in different mice strains. Recently we solved a crystal structure of the C-type lectin-like domain of a homologous protein, NKR-P1A, using X-ray crystallography and also described the strategy for rapid characterization of the protein conformation in solution. This procedure utilized chemical cross-linking, hydrogen/deuterium exchange, and molecular modeling. It was found that the solution structure differs from the crystal structure in the conformation of the loop region. The loop, detached from the protein compact core in the crystal structure, is closely attached to the core of the protein insolution. Here we present and interpret the solution structure of the C-type lectin-like domain of NKR-P1C using chemical cross-linking and molecular modeling. The validation of the model and conformation of the loop region in NKR-P1C we

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical chemistry

ISSN

0003-2700

e-ISSN

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Volume of the periodical

85

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1597-1604

UT code for WoS article

000314676100051

EID of the result in the Scopus database

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