The Camelina aquaporin CsPIP2;1 is regulated by phosphorylation at Ser273, but not at Ser277, of the C-terminus and is involved in salt- and drought-stress responses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F14%3A43887255" target="_blank" >RIV/60076658:12310/14:43887255 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.jplph.2014.06.009" target="_blank" >http://dx.doi.org/10.1016/j.jplph.2014.06.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jplph.2014.06.009" target="_blank" >10.1016/j.jplph.2014.06.009</a>

Result language

angličtina

Original language name

The Camelina aquaporin CsPIP2;1 is regulated by phosphorylation at Ser273, but not at Ser277, of the C-terminus and is involved in salt- and drought-stress responses

Original language description

Aquaporin (AQP) proteins are involved in water homeostasis in cells at all taxonomic levels of life. Phosphorylation of some AQPs has been proposed to regulate water permeability via gating of the channel itself. We analyzed plasma membrane intrinsic proteins (PIP) from Camelina and characterized their biological functions under both stressful and favorable conditions. A three-dimensional theoretical model of the Camelina AQP proteins was built by homology modeling which could prove useful in further functional characterization of AQPs. CsPIP2;1 was strongly and constitutively expressed in roots and leaves of Camelina, suggesting that this gene is related to maintenance of homeostasis during salt and drought stresses. CsPIP2s exhibited water channel activity in Xenopus oocytes. We then examined the roles of CsPIP2;1 phosphorylation at Ser273 and Ser277 in the regulation of water permeability using phosphorylation mutants. A single deletion strain of CsPIP2;1 was generated to serve as t

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

DA - Hydrology and limnology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Physiology

ISSN

0176-1617

e-ISSN

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Volume of the periodical

171

Issue of the periodical within the volume

15

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

1401-1412

UT code for WoS article

000345631000011

EID of the result in the Scopus database

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