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Evaluation of the diet of a carabid predator using fluorescent marking of prey

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F15%3A43890169" target="_blank" >RIV/60076658:12310/15:43890169 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.eje.cz/pdfs/eje/2015/03/11.pdf" target="_blank" >http://www.eje.cz/pdfs/eje/2015/03/11.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.14411/eje.2015.054" target="_blank" >10.14411/eje.2015.054</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Evaluation of the diet of a carabid predator using fluorescent marking of prey

  • Original language description

    We present a UV-fluorescent dye-based prey marking technique, using three different dyes, with later detection of the dyes in the guts of predators using a micro-plate fluorescent reader. By using this new method of detecting dyes based on their different excitation and emission characteristics it is possible to simultaneously screen 96 samples for multiple fluorescent-markers in less than 30 minutes. The marking is stable, cheap, non-toxic and had no effect on the choice of the carabid predator Pterostichus melanarius between marked and unmarked fly larvae (Musca domestica). Different fluorescent dyes provide variable detection intervals up to 100% detectability at 96 h post-ingestion by this predator. The simple marking, extraction and detection methods presented could be used in food web research to map individual trophic links and predator preference for different types of prey.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EH - Ecology - communities

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA526%2F09%2F1249" target="_blank" >GA526/09/1249: Significance of scavenging on invertebrate carcases in horticultural and agricultural landscape</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    European Journal of Entomology

  • ISSN

    1210-5759

  • e-ISSN

  • Volume of the periodical

    112

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    CZ - CZECH REPUBLIC

  • Number of pages

    9

  • Pages from-to

    477-485

  • UT code for WoS article

    000360715400011

  • EID of the result in the Scopus database