Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F15%3A43890213" target="_blank" >RIV/60076658:12310/15:43890213 - isvavai.cz</a>
Alternative codes found
RIV/67179843:_____/15:00452881
Result on the web
<a href="http://scripts.iucr.org/cgi-bin/paper?S2053230X15020270" target="_blank" >http://scripts.iucr.org/cgi-bin/paper?S2053230X15020270</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S2053230X15020270" target="_blank" >10.1107/S2053230X15020270</a>
Alternative languages
Result language
angličtina
Original language name
Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum
Original language description
The glyceraldehyde dehydrogenase from Thermoplasma acidophilum (TaAlDH) is a microbial enzyme that catalyzes the oxidation of D-glyceraldehyde to D-glycerate in the artificial enzyme cascade designed for the conversion of glucose to the organic solventsisobutanol and ethanol. Various mutants of TaAlDH were constructed by a random approach followed by site-directed and saturation mutagenesis in order to improve the properties of the enzyme that are essential for its functioning within the cascade. Two enzyme variants, wild-type TaAlDH (TaAlDHwt) and an F34M+S405N variant (TaAlDH F34M+S405N), were successfully crystallized. Crystals of TaAlDHwt belonged to the monoclinic space group P1211 with eight molecules per asymmetric unit and diffracted to a resolution of 1.95 angstrom. TaAlDH F34M+S405N crystallized in two different space groups: triclinic P1 with 16 molecules per asymmetric unit and monoclinic C121 with four molecules per asymmetric unit. These crystals diffracted to resolution
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta Crystallographica Section F-Structural Biology and Communications
ISSN
2053-230X
e-ISSN
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Volume of the periodical
71
Issue of the periodical within the volume
DEC 2015
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
1475-1480
UT code for WoS article
000369376500006
EID of the result in the Scopus database
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