Between sexual and apomictic: unexpectedly variable sporogenesis and production of viable polyhaploids in the pentaploid fern of the Dryopteris affinis agg. (Dryopteridaceae)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890568" target="_blank" >RIV/60076658:12310/16:43890568 - isvavai.cz</a>

Result on the web

<a href="http://aob.oxfordjournals.org/content/117/1/97" target="_blank" >http://aob.oxfordjournals.org/content/117/1/97</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/aob/mcv152" target="_blank" >10.1093/aob/mcv152</a>

Result language

angličtina

Original language name

Between sexual and apomictic: unexpectedly variable sporogenesis and production of viable polyhaploids in the pentaploid fern of the Dryopteris affinis agg. (Dryopteridaceae)

Original language description

Background and Aims In ferns, apomixis is an important mode of asexual reproduction. Although the mechanisms of fern reproduction have been studied thoroughly, most previous work has focused on cases in which ferns reproduce either exclusively sexually or exclusively asexually. Reproduction of ferns with potentially mixed systems and inheritance of apomixis remains largely unknown. This study addresses reproduction of the pentaploid Dryopteris x critica, a hybrid of triploid apomictic D. borreri and tetraploid sexual D. filix-mas. Methods Spore size, abortion percentage and number of spores per sporangium were examined in pentaploid plants of D. x critica grown in an experimental garden. The sporangial content of leaf segments was cultivated on an agar medium, and DNA ploidy levels were estimated by DAPI flow cytometry in 259 gametophytes or sporophytes arising from the F-2 generation of the pentaploid hybrid. Key Results The hybrid is partly fertile (89-94% of aborted spores) and shows unstable sporogenesis with sexual and apomictic reproduction combined. The number of spores per sporangium varied from approx. 31 to 64. Within a single sporangium it was possible to detect formation of either only aborted spores or various mixtures of aborted and well-developed reduced spores and unreduced diplospores. The spores germinated in viable gametophytes with two ploidy levels: pentaploid (5x, from unreduced spores) and half of that (approx. 2.5x, from reduced spores). Moreover, 2-15% of gametophytes (both 2.5x and 5x) formed a viable sporophyte of the same ploidy level due to apogamy. Conclusions This study documents the mixed reproductive mode of a hybrid between apomictic and sexual ferns. Both sexual reduced and apomictic unreduced spores can be produced by a single individual, and even within a single sporangium. Both types of spores give rise to viable F-2 generation gametophytes and sporophytes.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EH - Ecology - communities

OECD FORD branch

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Project

<a href="/en/project/GB14-36079G" target="_blank" >GB14-36079G: Plant diversity analysis and synthesis centre (PLADIAS)</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Annals of Botany

ISSN

0305-7364

e-ISSN

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Volume of the periodical

117

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

10

Pages from-to

97-106

UT code for WoS article

000369996700008

EID of the result in the Scopus database

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