Tracing nitrogen flow in a root-hemiparasitic association by foliar stable-isotope labelling

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890631" target="_blank" >RIV/60076658:12310/16:43890631 - isvavai.cz</a>

Result on the web

<a href="http://www.ingentaconnect.com/content/botbel/plecevo/2016/00000149/00000001/art00005" target="_blank" >http://www.ingentaconnect.com/content/botbel/plecevo/2016/00000149/00000001/art00005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5091/plecevo.2016.1112" target="_blank" >10.5091/plecevo.2016.1112</a>

Result language

angličtina

Original language name

Tracing nitrogen flow in a root-hemiparasitic association by foliar stable-isotope labelling

Original language description

Background and aims - The resource flows in the host-hemiparasite association have been frequently studied by applying stable isotope techniques. However, these methods of artificial labelling required sophisticated equipment preventing their application to field experiments. Here, we aimed to test the applicability of the (NC)-N-15-C-13-urea foliar brushing method in tracing the resource flows between a root hemiparasite, Rhinanthus major, and a host, Triticum aestivum. In addition, the dynamics of the label movement was examined in order to provide an estimate of the most appropriate harvesting time. Methods - Double-labelled urea (98 atom % N-15, 99 atom % C-13) solution (2 g dm(-3)) was applied on host plants grown with hemiparasites by a single foliar brushing. Above- and belowground biomass of both species was harvested 3, 7, and 14 d after host labelling and its isotopic composition was analyzed. Final isotopic enrichment of biomass was expressed as the atom percent difference between labelled samples and the mean of corresponding controls. Key results - Our results showed that a single leaf-brushing with (NC)-N-15-C-13-urea provided sufficiently N-15-labelled plant material, but it was insufficient to shift the natural abundance of C-13 in both species. Similar N-15 values were found for the host and hemiparasite biomass already 3 d after labelling, but the N-15 enrichment of attached hemiparasite significantly increased in time. Within a week, N-15-label gradually dispersed into the host tissues and was simultaneously transferred into the hemiparasite via the root connections. Conclusions - We present foliar brushing by N-15-urea as a simple and precise labelling method, which can be widely applied in both greenhouse and field experiments to examine the nitrogen flows between root hemiparasites and their host species.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

<a href="/en/project/GP14-26779P" target="_blank" >GP14-26779P: Searching for the Achilles' heel of Calamagrostis epigejos. Can we suppress the expansive grass by hemiparasitic Rhinanthus species?</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Ecology and Evolution

ISSN

2032-3913

e-ISSN

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Volume of the periodical

149

Issue of the periodical within the volume

1

Country of publishing house

BE - BELGIUM

Number of pages

6

Pages from-to

39-44

UT code for WoS article

000371196200005

EID of the result in the Scopus database

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