CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F16%3A43890959" target="_blank" >RIV/60076658:12310/16:43890959 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/16:00470774
Result on the web
<a href="https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcw115" target="_blank" >https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcw115</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/pcp/pcw115" target="_blank" >10.1093/pcp/pcw115</a>

Result language
angličtina
Original language name
CyanoP is Involved in the Early Steps of Photosystem II Assembly in the Cyanobacterium Synechocystis sp PCC 6803
Original language description
Although the PSII complex is highly conserved in cyanobacteria and chloroplasts, the PsbU and PsbV subunits stabilizing the oxygen-evolving Mn4CaO5 cluster in cyanobacteria are absent in chloroplasts and have been replaced by the PsbP and PsbQ subunits. There is, however, a distant cyanobacterial homolog of PsbP, termed CyanoP, of unknown function. Here we show that CyanoP plays a role in the early stages of PSII biogenesis in Synechocystis sp. PCC 6803. CyanoP is present in the PSII reaction center assembly complex (RCII) lacking both the CP47 and CP43 modules and binds to the smaller D2 module. A small amount of larger PSII core complexes co-purifying with FLAG-tagged CyanoP indicates that CyanoP can accompany PSII on most of its assembly pathway. A role in biogenesis is supported by the accumulation of unassembled D1 precursor and impaired formation of RCII in a mutant lacking CyanoP. Interestingly, the pull-down preparations of CyanoP-FLAG from a strain lacking CP47 also contained PsbO, indicating engagement of this protein with PSII at a much earlier stage in assembly than previously assumed.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)