Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895397" target="_blank" >RIV/60076658:12310/17:43895397 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/17:00100222

Result on the web

<a href="http://ac.els-cdn.com/S0045653516317179/1-s2.0-S0045653516317179-main.pdf?_tid=44a4ce56-9c71-11e7-90f2-00000aacb362&acdnat=1505739775_10338501d3e7eb56199555215a745d71" target="_blank" >http://ac.els-cdn.com/S0045653516317179/1-s2.0-S0045653516317179-main.pdf?_tid=44a4ce56-9c71-11e7-90f2-00000aacb362&acdnat=1505739775_10338501d3e7eb56199555215a745d71</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2016.12.006" target="_blank" >10.1016/j.chemosphere.2016.12.006</a>

Result language

angličtina

Original language name

Phytoestrogens and sterols in waters with cyanobacterial blooms - Analytical methods and estrogenic potencies

Original language description

Compounds with estrogenic potencies and their adverse effects in surface waters have received much attention. Both anthropogenic and natural compounds contribute to overall estrogenic activity in freshwaters. Recently, estrogenic potencies were also found to be associated with cyanobacteria and their blooms in surface waters. The present study developed and compared the solid phase extraction and LC-MS/MS analytical approaches for determination of phytoestrogens (8 flavonoids - biochanin A, coumestrol, daidzein, equol, formononetin, genistein, naringenin, apigenin and 5 sterols - ergosterol, (beta-sitosterol, stigmasterol, campesterol, brassicasterol) and cholesterol in water. The method was used for analyses of samples collected in stagnant water bodies dominated by different cyanobacterial species. Concentrations of individual flavonoids ranged from below the limit of detection to 3.58 ng/L. Sterols were present in higher amounts up to 2.25 mu g/L. Biological potencies of these phytoestrogens in vitro were characterized using the hER alpha-HeLa-9903 cell line. The relative estrogenic potencies (compared to model estrogen - 17 beta-estradiol) of flavonoids ranged from 2.25E-05 to 1.26E-03 with coumestrol being the most potent. None of the sterols elicited estrogenic response in the used bioassay. Estrogenic activity was detected in collected field water samples (maximum effect corresponding to 2.07 ng/L of 17 beta-estradiol equivalents, transcriptional assay). At maximum phytoestrogens accounted for only 1.56 pg/L of 17 beta-estradiol equivalents, contributing maximally 8.5% of the total estrogenicity of the water samples. Other compounds therefore, most likely of anthropogenic origin such as steroid estrogens, are probably the major drivers of total estrogenic effects in these surface waters.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10618 - Ecology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemosphere

ISSN

0045-6535

e-ISSN

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Volume of the periodical

170

Issue of the periodical within the volume

MAR 2017

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

104-112

UT code for WoS article

000392897100013

EID of the result in the Scopus database

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