Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F17%3A43895743" target="_blank" >RIV/60076658:12310/17:43895743 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/ppl.12508/epdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/ppl.12508/epdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ppl.12508" target="_blank" >10.1111/ppl.12508</a>
Alternative languages
Result language
angličtina
Original language name
Identification of an H2O2 permeable PIP aquaporin in barley and a serine residue promoting H2O2 transport
Original language description
A barley (Hordeum vulgare) plasma membrane type aquaporin, HvPIP2;5, was identified as an H2O2 permeable aquaporin among 21 barley and rice PIPs examined in the heterologous expression system using Saccharomyces cerevisiae. Four TIPs were also detected as H2O2-transporting aquaporins among 15 barley and rice TIPs. Influx of H2O2 into yeast cells expressing HvPIP2;5 was determined with a florescent-dye-based assay. Indirect immunofluorescence indicated that the expression of HvPIP2;5 protein was ubiquitous in root tissues, and was also weakly observed in leaf epidermal cells and cells in the vascular bundle. Point mutated variants of HvPIP2;5 were generated by the site-directed mutagenesis. Growth assays of yeast cells expressing these mutated HvPIP2;5 proteins suggested that Ser-126 in HvPIP2;5 has a large impact on H2O2 transport with a minor influence on the HvPIP2;5-mediated water transport.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
<a href="/en/project/EE2.3.30.0006" target="_blank" >EE2.3.30.0006: The Creation of Postdoc Positions at the University of South Bohemia and the Support of Itnersectoral Mobility by Expert Stays at the Foreign Leading R&D Institutions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Physiologia Plantarum
ISSN
0031-9317
e-ISSN
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Volume of the periodical
159
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
120-128
UT code for WoS article
000390650500009
EID of the result in the Scopus database
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