Ultrafast multi-pulse transient absorption spectroscopy of fucoxanthin chlorophyll a protein from Phaeodactylum tricomutum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897246" target="_blank" >RIV/60076658:12310/18:43897246 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/18:00495159

Result on the web

<a href="https://reader.elsevier.com/reader/sd/AE632B7C93732B33EF877DB6EDEDA8049E50983AA4EAFC09D0C94461E8DA11AE6895F575B95B6BB4B1E8D6F0B7235544" target="_blank" >https://reader.elsevier.com/reader/sd/AE632B7C93732B33EF877DB6EDEDA8049E50983AA4EAFC09D0C94461E8DA11AE6895F575B95B6BB4B1E8D6F0B7235544</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbabio.2018.02.011" target="_blank" >10.1016/j.bbabio.2018.02.011</a>

Result language

angličtina

Original language name

Ultrafast multi-pulse transient absorption spectroscopy of fucoxanthin chlorophyll a protein from Phaeodactylum tricomutum

Original language description

We have applied femtosecond transient absorption spectroscopy in pump-probe and pump-dump-probe regimes to study energy transfer between fucoxanthin and Chl a in fucoxanthin-Chl a complex from the pennate diatom Phaeodactylum tricornutum. Experiments were carried out at room temperature and 77 K to reveal temperature dependence of energy transfer. At both temperatures, the ultrafast (&lt; 100 fs) energy transfer channel from the fucoxanthin S-2 state is active and is complemented by the second pathway via the combined S-1/ICT state. The S-1/ICT-Chl a pathway has two channels, the fast one characterized by sub-picosecond energy transfer, and slow having time constants of 4.5 ps at room temperature and 6.6 ps at 77 K. The overall energy transfer via the S-1/ICT is faster at 77 K, because the fast component gains amplitude upon lowering the temperature. The pump dump-probe regime, with the dump pulse centered in the spectral region of ICT stimulated emission at 950 nm and applied at 2 ps after excitation, proved that the S-1 and ICT states of fucoxanthin in FCP are individual, yet coupled entities. Analysis of the pump-dump-probe data suggested that the main energy donor in the slow S-1/ICT-Chl a route is the S-1 part of the S-1/ICT potential surface.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

—

Volume of the periodical

1859

Issue of the periodical within the volume

5

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

357-365

UT code for WoS article

000430881200006

EID of the result in the Scopus database

2-s2.0-85043391297