Molecular Origin of Photoprotection in Cyanobacteria Probed by Watermarked Femtosecond Stimulated Raman Spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897247" target="_blank" >RIV/60076658:12310/18:43897247 - isvavai.cz</a>
Alternative codes found
RIV/68378271:_____/18:00501677 RIV/61388971:_____/18:00501677
Result on the web
<a href="http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5942868&blobtype=pdf" target="_blank" >http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC5942868&blobtype=pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jpclett.8b00663" target="_blank" >10.1021/acs.jpclett.8b00663</a>
Alternative languages
Result language
angličtina
Original language name
Molecular Origin of Photoprotection in Cyanobacteria Probed by Watermarked Femtosecond Stimulated Raman Spectroscopy
Original language description
Photoprotection is fundamental in photosynthesis to avoid oxidative photodamage upon excess light exposure. Excited chlorophylls (Chl) are quenched by carotenoids, but the precise molecular origin remains controversial. The cyanobacterial HliC protein belongs to the Hlip family ancestral to plant light-harvesting complexes, and binds Chl a and beta-carotene in 2:1 ratio. We analyzed HliC by watermarked femtosecond stimulated Raman spectroscopy to follow the time evolution of its vibrational modes. We observed a 2 ps rise of the C=C stretch band of the 2A(g)(-) (S-1) state of beta- carotene upon CM a excitation, demonstrating energy transfer quenching and fast excess-energy dissipation. We detected two distinct beta-carotene conformers by the C=C stretch frequency of the 2A(g)(-) (S-1) state, but only the beta-carotene whose 2A(g)(-) energy level is significantly lowered and has a lower C=C stretch frequency is involved in quenching. It implies that the low carotenoid S-1 energy that results from specific pigment-protein or pigment-pigment interactions is the key property for creating a dissipative energy channel. We conclude that watermarked femtosecond stimulated Raman spectroscopy constitutes a promising experimental method to assess energy transfer and quenching mechanisms in oxygenic photosynthesis.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
ISSN
1948-7185
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
1788-1792
UT code for WoS article
000429626900050
EID of the result in the Scopus database
2-s2.0-85045009663