IrC2/Bf - A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897383" target="_blank" >RIV/60076658:12310/18:43897383 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00498835 RIV/61388971:_____/18:00489065
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0145305X17304755?token=7094CB1FBFB3FC795CA54347A7AA449EB1AE0E3A4E4FF2415775C316FC7C63B64D5FF539111A57859F6FE2D9799D15D8" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0145305X17304755?token=7094CB1FBFB3FC795CA54347A7AA449EB1AE0E3A4E4FF2415775C316FC7C63B64D5FF539111A57859F6FE2D9799D15D8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.dci.2017.10.012" target="_blank" >10.1016/j.dci.2017.10.012</a>
Alternative languages
Result language
angličtina
Original language name
IrC2/Bf - A yeast and Borrelia responsive component of the complement system from the hard tick Ixodes ricinus
Original language description
Ticks possess components of a primordial complement system that presumably play a role in the interaction of the tick immune system with tick-borne pathogens and affect their transmission. Here we characterized a novel complement component, tagged as IrC2/Bf, from the hard tick Ixodes ricinus, the principal vector of Lyme disease in Europe. IrC2/Bf is a multi-domain molecule composed of 5-7 CCP modules, varied by alternative splicing, followed by a von Willebrand factor A domain and a C-terminal trypsin-like domain. The primary structure and molecular architecture of IrC2/Bf displays the closest homology to the C3-complement component convertases described in horseshoe crabs. The irc2/bf gene is mainly expressed in the tick fat body associated with the trachea and, as determined by western blotting, the protein is present in low amounts in tick hemolymph. Expression of irc2/bf mRNA was significantly up-regulated in response to the intra-hemocoelic injection of the yeast Candida albicans and all tested Borrelia sp. strains (B. burgdorferi NE5264, B. burgdorferi CB26, B. garinii IVISLB, B. afzelii CB43), but was not affected by injection of model Gram-negative and Gram-positive bacteria or the aseptic injection control. In-line with these results, RNAi-mediated silencing of irc2/bf inhibited phagocytosis of B. afzelii and C. albicans but not the other bacteria. Tissue expression profiles, specific responses to microbial challenges, and patterns of phagocytic phenotypes upon RNAi silencing observed for IrC2/Bf match well with the previously reported characteristics of I. ricinus C3-related molecule 1 (IrC3-1). Therefore we presume that IrC2/Bf functions as a convertase in the same complement activation pathway protecting ticks against yeast and Borrelia infection.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Developmental and Comparative Immunology
ISSN
0145-305X
e-ISSN
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Volume of the periodical
79
Issue of the periodical within the volume
FEB 2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
86-94
UT code for WoS article
000422811500010
EID of the result in the Scopus database
2-s2.0-85032298274