Leptomonas pyrrhocoris: Genomic insight into Parasite's Physiology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897401" target="_blank" >RIV/60076658:12310/18:43897401 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00497751 RIV/61988987:17310/18:A1901WFB
Result on the web
<a href="https://www.ingentaconnect.com/content/ben/cg/2018/00000019/00000002/art00010" target="_blank" >https://www.ingentaconnect.com/content/ben/cg/2018/00000019/00000002/art00010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2174/1389202918666170815143331" target="_blank" >10.2174/1389202918666170815143331</a>
Alternative languages
Result language
angličtina
Original language name
Leptomonas pyrrhocoris: Genomic insight into Parasite's Physiology
Original language description
Background: Leptomonas pyrrhocoris is a parasite of the firebug Pyrrhocoris apterus. This flagellate has been recently proposed as a model species for studying different aspects of the biology of monoxenous trypanosomatids, including host - parasite interactions. During its life cycle L. pyrrhocoris never tightly attaches to the epithelium of the insect gut. In contrast, its dixenous relatives (Leishmania spp.) establish a stable infection via attachment to the intestinal walls of their insect hosts. Material and Methods: This process is mediated by chemical modifications of the cell surface lipophosphoglycans. In our study we tested whether the inability of L. pyrrhocoris to attach to the firebug's midgut is associated with the absence of these glycoconjugates. We also analyzed evolution of the proteins involved in proper lipophosphoglycan assembly, cell attachment and establishment of a stable infection in L. pyrrhocoris, L. seymouri, and Leishmania spp. Our comparative analysis demonstrated differences in SCG/L/R repertoire between the two parasite subgenera, Leishmania and Viannia, which may be related to distinct life strategies in various Leishmania spp. The genome of L. pyrrhocoris encodes 6 SCG genes, all of which are quite divergent from their orthologs in the genus Leishmania. Using direct probing with an antibody recognizing the beta-Gal side chains of lipophosphoglycans, we confirmed that these structures are not synthesized in L. pyrrhocoris. Conclusion: We conclude that either the SCG enzymes are not active in this species (similarly to SCG5/7 in L. major), or they possess a different biochemical activity.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Genomics
ISSN
1389-2029
e-ISSN
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Volume of the periodical
19
Issue of the periodical within the volume
2
Country of publishing house
AE - UNITED ARAB EMIRATES
Number of pages
7
Pages from-to
150-156
UT code for WoS article
000419990300009
EID of the result in the Scopus database
2-s2.0-85042793007