Cryptophyta as major bacterivores in freshwater summer plankton
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897429" target="_blank" >RIV/60076658:12310/18:43897429 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00495058
Result on the web
<a href="https://www.nature.com/articles/s41396-018-0057-5" target="_blank" >https://www.nature.com/articles/s41396-018-0057-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41396-018-0057-5" target="_blank" >10.1038/s41396-018-0057-5</a>
Alternative languages
Result language
angličtina
Original language name
Cryptophyta as major bacterivores in freshwater summer plankton
Original language description
Small bacterivorous eukaryotes play a cardinal role in aquatic food webs and their taxonomic classification is currently a hot topic in aquatic microbial ecology. Despite increasing interest in their diversity, core questions regarding predator-prey specificity remain largely unanswered, e.g., which heterotrophic nanoflagellates (HNFs) are the main bacterivores in freshwaters and which prokaryotes support the growth of small HNFs. To answer these questions, we fed natural communities of HNFs from Rimov reservoir (Czech Republic) with five different bacterial strains of the ubiquitous betaproteobacterial genera Polynucleobacter and Limnohabitans. We combined amplicon sequencing and catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH) targeting eukaryotic 18 S rRNA genes to track specific responses of the natural HNF community to prey amendments. While amplicon sequencing provided valuable qualitative data and a basis for designing specific probes, the number of reads was insufficient to accurately quantify certain eukaryotic groups. We also applied a double-hybridization technique that allows simultaneous phylogenetic identification of both predator and prey. Our results show that community composition of HNFs is strongly dependent upon prey type. Surprisingly, Cryptophyta were the most abundant bacterivores, although this phylum has been so far assumed to be mainly autotrophic. Moreover, the growth of a small lineage of Cryptophyta (CRY1 clade) was strongly stimulated by one Limnohabitans strain in our experiment. Thus, our study is the first report that colorless Cryptophyta are major bacterivores in summer plankton samples and can play a key role in the carbon transfer from prokaryotes to higher trophic levels.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA13-00243S" target="_blank" >GA13-00243S: Unveiling life strategies of selected groups of planktonic Betaproteobacteria in relationship to carbon flow to higher trophic levels</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
I S M E Journal
ISSN
1751-7362
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
7
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
1668-1681
UT code for WoS article
000436623600004
EID of the result in the Scopus database
2-s2.0-85042224663