Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897433" target="_blank" >RIV/60076658:12310/18:43897433 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00497528
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0166685118301166?token=4FB73829BCF217372E9EF8D2B371813194762437E3B304F61E833F2789125A3608DDF3068938BDC8C7A1BE34B16B4B09" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0166685118301166?token=4FB73829BCF217372E9EF8D2B371813194762437E3B304F61E833F2789125A3608DDF3068938BDC8C7A1BE34B16B4B09</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.molbiopara.2018.08.003" target="_blank" >10.1016/j.molbiopara.2018.08.003</a>

Result language
angličtina
Original language name
Development of microsatellite loci in zoonotic tapeworm Dibothriocephalus latus (Linnaeus, 1758), Luhe, 1899 (syn. Diphyllobothrium latum) using microsatellite library screening
Original language description
The broad fish tapeworm Dibothriocephalus lams is a causative agent of human food-borne disease called diphyllobothriosis. Medical importance, scattered geographical distribution and unknown origin of D. latus in Europe and North America make this species to be an interesting model for population genetics. Microsatellite markers were originally designed by library screening using NGS approach and validated as tools for future studies on population genetics of D. latus. Out of 122 candidates selected after NGS analysis, 110 yielded PCR products of the expected size, and in 78 of them, a declared repetitive motif was confirmed by Sanger sequencing. After the fragment analysis, six loci were proved to be polymorphic and tested for observed (Ho) and expected (He) heterozygosity, and deviations from Hardy-Weinberg equilibrium (HWE). They promise future application in studies on genetic interrelationships, origin and migratory routes of this medically important emerging tapeworm.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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