Binding of pigments to the cyanobacterial high-light-inducible protein HliC

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897486" target="_blank" >RIV/60076658:12310/18:43897486 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/18:00491694
Result on the web
<a href="https://link.springer.com/content/pdf/10.1007%2Fs11120-017-0475-7.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs11120-017-0475-7.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11120-017-0475-7" target="_blank" >10.1007/s11120-017-0475-7</a>

Result language
angličtina
Original language name
Binding of pigments to the cyanobacterial high-light-inducible protein HliC
Original language description
Cyanobacteria possess a family of one-helix high-light-inducible proteins (HLIPs) that are widely viewed as ancestors of the light-harvesting antenna of plants and algae. HLIPs are essential for viability under various stress conditions, although their exact role is not fully understood. The unicellular cyanobacterium Synechocystis sp. PCC 6803 contains four HLIPs named HliA-D, and HliD has recently been isolated in a small protein complex and shown to bind chlorophyll and beta-carotene. However, no HLIP has been isolated and characterized in a pure form up to now. We have developed a protocol to purify large quantities of His-tagged HliC from an engineered Synechocystis strain. Purified His-HliC is a pigmented homo-oligomer and is associated with chlorophyll and beta-carotene with a 2:1 ratio. This differs from the 3:1 ratio reported for HliD. Comparison of these two HLIPs by resonance Raman spectroscopy revealed a similar conformation for their bound beta-carotenes, but clear differences in their chlorophylls. We present and discuss a structural model of HliC, in which a dimeric protein binds four chlorophyll molecules and two beta-carotenes.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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