Antenna proton sensitivity determines photosynthetic light harvesting strategy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897490" target="_blank" >RIV/60076658:12310/18:43897490 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/18:00494118

Result on the web

<a href="https://academic.oup.com/jxb/article/69/18/4483/5046136" target="_blank" >https://academic.oup.com/jxb/article/69/18/4483/5046136</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jxb/ery240" target="_blank" >10.1093/jxb/ery240</a>

Result language

angličtina

Original language name

Antenna proton sensitivity determines photosynthetic light harvesting strategy

Original language description

Photoprotective non-photochemical quenching (NPQ) represents an effective way to dissipate the light energy absorbed in excess by most phototrophs. It is often claimed that NPQ formation/relaxation kinetics are determined by xanthophyll composition. We, however, found that, for the alveolate alga Chromera velia, this is not the case. In the present paper, we investigated the reasons for the constitutive high rate of quenching displayed by the alga by comparing its light harvesting strategies with those of a model phototroph, the land plant Spinacia oleracea. Experimental results and in silico studies support the idea that fast quenching is due not to xanthophylls, but to intrinsic properties of the Chromera light harvesting complex (CLH) protein, related to amino acid composition and protein folding. The pK(a) for CLH quenching was shifted by 0.5 units to a higher pH compared with higher plant antennas (light harvesting complex II; LHCII). We conclude that, whilst higher plant LHCIIs are better suited for light harvesting, CLHs are &apos;natural quenchers&apos; ready to switch into a dissipative state. We propose that organisms with antenna proteins intrinsically more sensitive to protons, such as C. velia, carry a relatively high concentration of violaxanthin to improve their light harvesting. In contrast, higher plants need less violaxanthin per chlorophyll because LHCII proteins are more efficient light harvesters and instead require co-factors such as zeaxanthin and PsbS to accelerate and enhance quenching.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Experimental Botany

ISSN

0022-0957

e-ISSN

—

Volume of the periodical

69

Issue of the periodical within the volume

18

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

4483-4493

UT code for WoS article

000442309600021

EID of the result in the Scopus database

2-s2.0-85055562082