Carbonate-sensitive phytotransferrin controls high-affinity iron uptake in diatoms

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897564" target="_blank" >RIV/60076658:12310/18:43897564 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/18:00498760

Result on the web

<a href="https://www.nature.com/articles/nature25982" target="_blank" >https://www.nature.com/articles/nature25982</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/nature25982" target="_blank" >10.1038/nature25982</a>

Result language

angličtina

Original language name

Carbonate-sensitive phytotransferrin controls high-affinity iron uptake in diatoms

Original language description

In vast areas of the ocean, the scarcity of iron controls the growth and productivity of phytoplankton(1,2). Although most dissolved iron in the marine environment is complexed with organic molecules(3), picomolar amounts of labile inorganic iron species (labile iron) are maintained within the euphotic zone(4) and serve as an important source of iron for eukaryotic phytoplankton and particularly for diatoms(5). Genome-enabled studies of labile iron utilization by diatoms have previously revealed novel iron responsive transcripts(6,7), including the ferric iron-concentrating protein ISIP2A(8), but the mechanism behind the acquisition of picomolar labile iron remains unknown. Here we show that ISIP2A is a phytotransferrin that independently and convergently evolved carbonate ion-coordinated ferric iron binding. Deletion of ISIP2A disrupts high-affinity iron uptake in the diatom Phaeodactylum tricornutum, and uptake is restored by complementation with human transferrin. ISIP2A is internalized by endocytosis, and manipulation of the seawater carbonic acid system reveals a second order dependence on the concentrations of labile iron and carbonate ions. In P. tricornutum, the synergistic interaction of labile iron and carbonate ions occurs at environmentally relevant concentrations, revealing that carbonate availability co-limits iron uptake. Phytotransferrin sequences have a broad taxonomic distribution(8) and are abundant in marine environmental genomic datasets(9,10), suggesting that acidification-driven declines in the concentration of seawater carbonate ions will have a negative effect on this globally important eukaryotic iron acquisition mechanism.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

<a href="/en/project/GA15-17643S" target="_blank" >GA15-17643S: A search for the origin of exosymbiont</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nature

ISSN

0028-0836

e-ISSN

—

Volume of the periodical

555

Issue of the periodical within the volume

7697

Country of publishing house

GB - UNITED KINGDOM

Number of pages

20

Pages from-to

534-"+"

UT code for WoS article

000427937900056

EID of the result in the Scopus database

2-s2.0-85044265918