Massive mitochondrial DNA content in diplonemid and kinetoplastid protists

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897635" target="_blank" >RIV/60076658:12310/18:43897635 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/18:00498753

Result on the web

<a href="https://iubmb.onlinelibrary.wiley.com/doi/epdf/10.1002/iub.1894" target="_blank" >https://iubmb.onlinelibrary.wiley.com/doi/epdf/10.1002/iub.1894</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/iub.1894" target="_blank" >10.1002/iub.1894</a>

Result language

angličtina

Original language name

Massive mitochondrial DNA content in diplonemid and kinetoplastid protists

Original language description

The mitochondrial DNA of diplonemid and kinetoplastid protists is known for its suite of bizarre features, including the presence of concatenated circular molecules, extensive trans-splicing and various forms of RNA editing. Here we report on the existence of another remarkable characteristic: hyper-inflated DNA content. We estimated the total amount of mitochondrial DNA in four kinetoplastid species (Trypanosoma brucei, Trypanoplasma borreli, Cryptobia helicis, and Perkinsela sp.) and the diplonemid Diplonema papillatum. Staining with 4 &apos;,6-diamidino-2-phenylindole and RedDot1 followed by color deconvolution and quantification revealed massive inflation in the total amount of DNA in their organelles. This was further confirmed by electron microscopy. The most extreme case is the similar to 260 Mbp of DNA in the mitochondrion of Diplonema, which greatly exceeds that in its nucleus; this is, to our knowledge, the largest amount of DNA described in any organelle. Perkinsela sp. has a total mitochondrial DNA content similar to 6.6x greater than its nuclear genome. This mass of DNA occupies most of the volume of the Perkinsela cell, despite the fact that it contains only six protein-coding genes. Why so much DNA? We propose that these bloated mitochondrial DNAs accumulated by a ratchet-like process. Despite their excessive nature, the synthesis and maintenance of these mtDNAs must incur a relatively low cost, considering that diplonemids are one of the most ubiquitous and speciose protist groups in the ocean. (c) 2018 IUBMB Life

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

IUBMB Life

ISSN

1521-6543

e-ISSN

—

Volume of the periodical

70

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

1267-1274

UT code for WoS article

000450862000011

EID of the result in the Scopus database

2-s2.0-85054503156