Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897679" target="_blank" >RIV/60076658:12310/18:43897679 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0960852418311684?token=93FB0626F1A05E26E71B3338E3311702FDEEFEA86E8676528AD1D781E627011CC789521D10643131AA5829B2F461FE01" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0960852418311684?token=93FB0626F1A05E26E71B3338E3311702FDEEFEA86E8676528AD1D781E627011CC789521D10643131AA5829B2F461FE01</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.biortech.2018.08.064" target="_blank" >10.1016/j.biortech.2018.08.064</a>

Result language
angličtina
Original language name
Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept
Original language description
Grass silage as a renewable feedstock for an integrated biorefinery includes nutrients and carbon sources directly available in the press juice (PJ) and in lignocellulosic saccharides from the plant framework. Here, a novel two-stage fed-batch fermentation process for biosynthesis of poly-3-hydroxybutyrate (PHB) by Cupriavidus necator DSM 531 is presented. For bacterial growth, nutrient-rich PJ was employed as a fermentation medium, without any supplements. Saccharides derived from the mechano-enzymatic hydrolysis of the press cake (PC) were subjected to a lactic acid fermentation process, before the fermentation products were fed into the polymer accumulation phase. By combination of pH-stat feeding and cell recycling, the PHB content in 22 g L-1 total-dry cells reached 39% after 32 h of cultivation. Using mimicked hydrolyzate of diluted PJ artificially supplemented with glucose and xylose, the resulting cell dry weight of 21 g L-1 contained 42% PHB.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20802 - Bioremediation, diagnostic biotechnologies (DNA chips and biosensing devices) in environmental management

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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