The reduced genome of Candidatus Kinetoplastibacterium sorsogonicusi, the endosymbiont of Kentomonas sorsogonicus (Trypanosomatidae): loss of the haem-synthesis pathway

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897706" target="_blank" >RIV/60076658:12310/18:43897706 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/18:00498811 RIV/61988987:17310/18:A1901X5C

Result on the web

<a href="https://www.cambridge.org/core/journals/parasitology/article/reduced-genome-of-candidatus-kinetoplastibacterium-sorsogonicusi-the-endosymbiont-of-kentomonas-sorsogonicus-trypanosomatidae-loss-of-the-haemsynthesis-pathway/4513A86CF1C593C863E7488D41093F1E#" target="_blank" >https://www.cambridge.org/core/journals/parasitology/article/reduced-genome-of-candidatus-kinetoplastibacterium-sorsogonicusi-the-endosymbiont-of-kentomonas-sorsogonicus-trypanosomatidae-loss-of-the-haemsynthesis-pathway/4513A86CF1C593C863E7488D41093F1E#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S003118201800046X" target="_blank" >10.1017/S003118201800046X</a>

Result language

angličtina

Original language name

The reduced genome of Candidatus Kinetoplastibacterium sorsogonicusi, the endosymbiont of Kentomonas sorsogonicus (Trypanosomatidae): loss of the haem-synthesis pathway

Original language description

Trypanosomatids of the genera Angomonas and Strigomonas (subfamily Strigomonadinae) have long been known to contain intracellular beta-proteobacteria, which provide them with many important nutrients such as haem, essential amino acids and vitamins. Recently, Kentomonas sorsogonicus, a divergent member of Strigomonadinae, has been described. Herein, we characterize the genome of its endosymbiont, Candidatus Kinetoplastibacterium sorsogonicusi. This genome is completely syntenic with those of other known Ca. Kinetoplastibacterium spp., but more reduced in size (similar to 742 kb, compared with 810-833 kb, respectively). Gene losses are not concentrated in any hot-spots but are instead distributed throughout the genome. The most conspicuous loss is that of the haem-synthesis pathway. For long, removing haemin from the culture medium has been a standard procedure in cultivating trypanosomatids isolated from insects; continued growth was considered as an evidence of endosymbiont presence. However, we demonstrate that, despite bearing the endosymbiont, K. sorsogonicus cannot grow in culture without haem. Thus, the traditional test cannot be taken as a reliable criterion for the absence or presence of endosymbionts in trypanosomatid flagellates. It remains unclear why the ability to synthesize such an essential compound was lost in Ca. K. sorsogonicusi, whereas all other known bacterial endosymbionts of trypanosomatids retain them.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology

ISSN

0031-1820

e-ISSN

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Volume of the periodical

145

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

1287-1293

UT code for WoS article

000443420600005

EID of the result in the Scopus database

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