Evaluating rodent experimental models for studies of Blastocystis ST1
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43897727" target="_blank" >RIV/60076658:12310/18:43897727 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00498801
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0014489418301784?token=FF0A5AC063C89195C2A28D105497F005E9B6CBD81FA0772FA7D7FF6AC79E7789BE9A7AAAA6814E32715D0E91B5A1C6A0" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0014489418301784?token=FF0A5AC063C89195C2A28D105497F005E9B6CBD81FA0772FA7D7FF6AC79E7789BE9A7AAAA6814E32715D0E91B5A1C6A0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exppara.2018.06.009" target="_blank" >10.1016/j.exppara.2018.06.009</a>
Alternative languages
Result language
angličtina
Original language name
Evaluating rodent experimental models for studies of Blastocystis ST1
Original language description
Blastocystis is a common inhabitant of the human gut, colonizing at least one billion people at a prevalence ranging from < 10% to 100% in healthy human populations globally. The majority of carriers remain asymptomatic, suggesting that Blastocystis is largely a commensal, though Blastocystis has also been implicated in disease in some people. However, there are no in vivo model systems in which to experimentally test the impact of Blastocystis on mammalian hosts and the gut ecosystem and determine which factors underlie these variable clinical outcomes. We evaluated a rat model for sustaining of a human-derived Blastocystis ST1 and assess colonization success and longevity. Because of the broad host range of Blastocystis, we compared the rat with three other rodent species to establish the reproducibility of our method. Blastocystis was introduced by esophageal gavage and colonization success evaluated by Blastocystis culture. Culture was also used to determine that all animals were negative prior to colonization and negative controls remain Blastocystis-free. In this study, Blastocystis ST1 established in 100% of the outbred rats (Rattus norvegicus) and gerbils (Meriones unguiculatus) challenged. Rats were colonized asymptomatically for more than one year, but Blastocystis ST1 was not transmitted between rats. Mus musculus strain CD1 and Mastomys coucha were not susceptible to Blastocystis ST1. Thus, rats appear to be a suitable in vivo model for studies of Blastocystis ST1, as do gerbils though testing was less extensive. This work lays the foundation for experimental work on the role of Blastocystis in health and disease.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental Parasitology
ISSN
0014-4894
e-ISSN
—
Volume of the periodical
191
Issue of the periodical within the volume
AUG 2018
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
55-61
UT code for WoS article
000441369900008
EID of the result in the Scopus database
2-s2.0-85049931024