Heterotrophic N-2-fixation contributes to nitrogen economy of a common wetland sedge, Schoenoplectus californicus
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F18%3A43898441" target="_blank" >RIV/60076658:12310/18:43898441 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/18:00495233
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pone.0195570" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0195570</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0195570" target="_blank" >10.1371/journal.pone.0195570</a>
Alternative languages
Result language
angličtina
Original language name
Heterotrophic N-2-fixation contributes to nitrogen economy of a common wetland sedge, Schoenoplectus californicus
Original language description
A survey of the ecological variability within 52 populations of Schoenoplectus californicus (C.A. Mey.) Sojak across its distributional range revealed that it is commonly found in nitrogen (N) limited areas, but rarely in phosphorus limited soils. We explored the hypothesis that S. californicus supplements its nitrogen demand by bacterial N-2-fixation processes associated with its roots and rhizomes. We estimated N-2-fixation of diazotrophs associated with plant rhizomes and roots from several locations throughout the species' range and conducted an experiment growing plants in zero, low, and high N additions. Nitrogenase activity in rhizomes and roots was measured using the acetylene reduction assay. The presence of diazotrophs was verified by the detection of the nifHgene. Nitrogenase activity was restricted to rhizomes and roots and it was two orders of magnitude higher in the latter plant organs (81 and 2032 nmol C2H4 g DW-1 d(-1), respectively). Correspondingly, 40x more nifH gene copies were found on roots compared to rhizomes. The proportion of the nifHgene copies in total bacterial DNA was positively correlated with the nitrogenase activity. In the experiment, the contribution of fixed N to the plant N content ranged from 13.8% to 32.5% among clones from different locations. These are relatively high values for a non-cultivated plant and justify future research on the link between N-fixing bacteria and S. californicus production.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS One
ISSN
1932-6203
e-ISSN
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Volume of the periodical
13
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
22
Pages from-to
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UT code for WoS article
000430660600016
EID of the result in the Scopus database
2-s2.0-85045952060