Laboratory acclimation to autumn-like conditions induces freeze tolerance in the spring field cricket Gryllus veletis (Orthoptera: Gryllidae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899141" target="_blank" >RIV/60076658:12310/19:43899141 - isvavai.cz</a>
Result on the web
<a href="https://reader.elsevier.com/reader/sd/pii/S0022191018303585?token=3E89D10F4801FC0C873C682CEDBF2B218C6B10890844AB149121C68D56C97D862AEF14EEE501B9F552AE72BB61A7AC24" target="_blank" >https://reader.elsevier.com/reader/sd/pii/S0022191018303585?token=3E89D10F4801FC0C873C682CEDBF2B218C6B10890844AB149121C68D56C97D862AEF14EEE501B9F552AE72BB61A7AC24</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jinsphys.2018.12.007" target="_blank" >10.1016/j.jinsphys.2018.12.007</a>
Alternative languages
Result language
angličtina
Original language name
Laboratory acclimation to autumn-like conditions induces freeze tolerance in the spring field cricket Gryllus veletis (Orthoptera: Gryllidae)
Original language description
Many temperate insects encounter temperatures low enough to freeze their body fluids. Remarkably, some insects are freeze-tolerant, surviving this internal ice formation. However, the mechanisms underlying freeze tolerance are not well-understood, in part due to a lack of tractable model organisms. We describe a novel laboratory model to study insect freeze tolerance, the spring field cricket Gryllus veletis (Orthopera: Gryllidae). Following acclimation to six weeks of decreasing temperature and photoperiod, G. veletis become freeze-tolerant, similar to those exposed to natural autumn conditions in London, Ontario, Canada. Acclimated crickets suppress their metabolic rate by c. 33%, and survive freezing for up to one week at -8 degrees C, and to temperatures as low as -12 degrees C. Freeze-tolerant G. veletis protect fat body cells from freeze injury in vivo, and fat body tissue from freeze-tolerant cricket survives brief freeze treatments when frozen ex vivo. Freeze-tolerant crickets freeze at c. -6 degrees C, which may be initiated by accumulation of ice-nucleating agents in hemolymph or gut tissue. Although we hypothesize that control of ice formation facilitates freeze tolerance, initiating ice formation at high subzero temperatures does not confer freeze tolerance on freeze-intolerant nymphs. Acclimation increases hemolymph osmolality from c. 400 to c. 650 mOsm, which may facilitate freeze tolerance by reducing ice content. Hemolymph ion concentrations do not change with acclimation, and we therefore predict that freeze-tolerant G. veletis elevate hemolymph osmolality by accumulating other molecules. Gryllus veletis is easily reared and manipulated in a controlled laboratory environment, and is therefore a suitable candidate for further investigating the mechanisms underlying freeze tolerance.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10616 - Entomology
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Insect Physiology
ISSN
0022-1910
e-ISSN
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Volume of the periodical
113
Issue of the periodical within the volume
FEB-MAR 2019
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
9-16
UT code for WoS article
000463121900002
EID of the result in the Scopus database
2-s2.0-85059654147