The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F19%3A43899427" target="_blank" >RIV/60076658:12310/19:43899427 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/19:00519639 RIV/61388963:_____/19:00519639
Result on the web
<a href="https://link.springer.com/content/pdf/10.1007%2Fs00018-019-03034-3.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs00018-019-03034-3.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00018-019-03034-3" target="_blank" >10.1007/s00018-019-03034-3</a>

Result language
angličtina
Original language name
The structure and function of Iristatin, a novel immunosuppressive tick salivary cystatin
Original language description
To successfully feed, ticks inject pharmacoactive molecules into the vertebrate host including cystatin cysteine protease inhibitors. However, the molecular and cellular events modulated by tick saliva remain largely unknown. Here, we describe and characterize a novel immunomodulatory cystatin, Iristatin, which is upregulated in the salivary glands of feeding Ixodes ricinus ticks. We present the crystal structure of Iristatin at 1.76 angstrom resolution. Purified recombinant Iristatin inhibited the proteolytic activity of cathepsins L and C and diminished IL-2, IL-4, IL-9, and IFN- production by different T-cell populations, IL-6 and IL-9 production by mast cells, and nitric oxide production by macrophages. Furthermore, Iristatin inhibited OVA antigen-induced CD4(+) T-cell proliferation and leukocyte recruitment in vivo and in vitro. Our results indicate that Iristatin affects wide range of anti-tick immune responses in the vertebrate host and may be exploitable as an immunotherapeutic.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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