Insect-Symbiont Gene Expression in the Midgut Bacteriocytes of a Blood-Sucking Parasite

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901137" target="_blank" >RIV/60076658:12310/20:43901137 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/20:00538246

Result on the web

<a href="https://academic.oup.com/gbe/article/12/4/429/5739960" target="_blank" >https://academic.oup.com/gbe/article/12/4/429/5739960</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/gbe/evaa032" target="_blank" >10.1093/gbe/evaa032</a>

Result language

angličtina

Original language name

Insect-Symbiont Gene Expression in the Midgut Bacteriocytes of a Blood-Sucking Parasite

Original language description

Animals interact with a diverse array of both beneficial and detrimental microorganisms. In insects, these symbioses in many cases allow feeding on nutritionally unbalanced diets. It is, however, still not clear how are obligate symbioses maintained at the cellular level for up to several hundred million years. Exact mechanisms driving host-symbiont interactions are only understood for a handful of model species and data on blood-feeding hosts with intracellular bacteria are particularly scarce. Here, we analyzed interactions between an obligately blood-sucking parasite of sheep, the louse fly Melophagus ovinus, and its obligate endosymbiont, Arsenophonus melophagi. We assembled a reference transcriptome for the insect host and used dual RNA-Seq with five biological replicates to compare expression in the midgut cells specialized for housing symbiotic bacteria (bacteriocytes) to the rest of the gut (foregut-hindgut). We found strong evidence for the importance of zinc in the system likely caused by symbionts using zinc-dependent proteases when acquiring amino acids, and for different immunity mechanisms controlling the symbionts than in closely related tsetse flies. Our results show that cellular and nutritional interactions between this blood-sucking insect and its symbionts are less intimate than what was previously found in most plant-sap sucking insects. This finding is likely interconnected to several features observed in symbionts in blood-sucking arthropods, particularly their midgut intracellular localization, intracytoplasmic presence, less severe genome reduction, and relatively recent associations caused by frequent evolutionary losses and replacements.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10618 - Ecology

Project

<a href="/en/project/GA18-07711S" target="_blank" >GA18-07711S: Ecological, genomic and metabolic processes accompanying adaptations of symbiotic bacteria and blood feeding insects.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome Biology and Evolution

ISSN

1759-6653

e-ISSN

—

Volume of the periodical

12

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

429-442

UT code for WoS article

000538703000016

EID of the result in the Scopus database

2-s2.0-85084272550