Excited-State Properties of Canthaxanthin in Cyanobacterial Carotenoid-Binding Proteins HCP2 and HCP3

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901337" target="_blank" >RIV/60076658:12310/20:43901337 - isvavai.cz</a>
Result on the web
<a href="https://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.0c03137" target="_blank" >https://pubs.acs.org/doi/pdf/10.1021/acs.jpcb.0c03137</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jpcb.0c03137" target="_blank" >10.1021/acs.jpcb.0c03137</a>

Result language
angličtina
Original language name
Excited-State Properties of Canthaxanthin in Cyanobacterial Carotenoid-Binding Proteins HCP2 and HCP3
Original language description
Recently a new family of carotenoproteins, homologues of the N-terminal domain of the orange carotenoid protein (NTD-OCP), have been identified in cyanobacteria. These homologues are called helical carotenoid proteins (HCPs) as they are all predicted to maintain the all-helical structure of the NTD- OCP and to bind carotenoids. Here, HCP2 and HCP3 isolated from the cyanobacterium Tolypothrix PCC 7601 were studied by ultrafast transient absorption spectroscopy to explore the excited-state dynamics of the bound carotenoid, canthaxanthin. The lowest excited state, S-1 of canthaxanthin in both HCPs yields a lifetime of 3.5 ps; it is thus shorter than for canthaxanthin in solution (4.5 ps). This is because of the longer effective conjugation of canthaxanthin in HCPs, as one of the terminal rings is in an s-trans configuration. Use of two different excitation wavelengths, 470 and 570 nm, revealed excitation wavelength dependent spectroscopic response. Additional excited-state absorption bands are observed after excitation at 470 nm for both HCPs, proving the presence of more than one ground state conformer.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10610 - Biophysics

Project
<a href="/en/project/GA18-21631S" target="_blank" >GA18-21631S: Ultrafast spectroscopy as a tool for elucidation the structure-function relationship in cyanobacterial carotenoid-binding proteins</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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