Age-related differences in the translational landscape of mammalian oocytes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901371" target="_blank" >RIV/60076658:12310/20:43901371 - isvavai.cz</a>

Alternative codes found

RIV/67985904:_____/20:00536356 RIV/00216208:11310/20:10421429

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/acel.13231" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/acel.13231</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/acel.13231" target="_blank" >10.1111/acel.13231</a>

Result language

angličtina

Original language name

Age-related differences in the translational landscape of mammalian oocytes

Original language description

Increasing maternal age in mammals is associated with poorer oocyte quality, involving higher aneuploidy rates and decreased developmental competence. Prior to resumption of meiosis, fully developed mammalian oocytes become transcriptionally silent until the onset of zygotic genome activation. Therefore, meiotic progression and early embryogenesis are driven largely by translational utilization of previously synthesized mRNAs. We report that genome-wide translatome profiling reveals considerable numbers of transcripts that are differentially translated in oocytes obtained from aged compared to young females. Additionally, we show that a number of aberrantly translated mRNAs in oocytes from aged females are associated with cell cycle. Indeed, we demonstrate that four specific maternal age-related transcripts (Sgk1,Castor1,AireandEg5) with differential translation rates encode factors that are associated with the newly forming meiotic spindle. Moreover, we report substantial defects in chromosome alignment and cytokinesis in the oocytes of young females, in which candidate CASTOR1 and SGK1 protein levels or activity are experimentally altered. Our findings indicate that improper translation of specific proteins at the onset of meiosis contributes to increased chromosome segregation problems associated with female ageing.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10602 - Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology

Project

<a href="/en/project/GA19-13491S" target="_blank" >GA19-13491S: In vitro oocyte culture vs in vivo oocyte development - is their physiology really comparable?</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aging Cell

ISSN

1474-9718

e-ISSN

—

Volume of the periodical

19

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

—

UT code for WoS article

000570994100001

EID of the result in the Scopus database

2-s2.0-85091109400