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EukRef-excavates: seven curated SSU ribosomal RNA gene databases

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901425" target="_blank" >RIV/60076658:12310/20:43901425 - isvavai.cz</a>

  • Alternative codes found

    RIV/60077344:_____/20:00538274 RIV/00216208:11310/20:10419237

  • Result on the web

    <a href="http://fdslive.oup.com/www.oup.com/pdf/production_in_progress.pdf" target="_blank" >http://fdslive.oup.com/www.oup.com/pdf/production_in_progress.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/database/baaa080" target="_blank" >10.1093/database/baaa080</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    EukRef-excavates: seven curated SSU ribosomal RNA gene databases

  • Original language description

    The small subunit ribosomal RNA (SSU rRNA) gene is a widely used molecular marker to study the diversity of life. Sequencing of SSU rRNA gene amplicons has become a standard approach for the investigation of the ecology and diversity of microbes. However, a well-curated database is necessary for correct classification of these data. While available for many groups of Bacteria and Archaea, such reference databases are absent for most eukaryotes. The primary goal of the EukRef project (eukref.org ) is to close this gap and generate well-curated reference databases for major groups of eukaryotes, especially protists. Here we present a set of EukRef-curated databases for the excavate protists-a large assemblage that includes numerous taxa with divergent SSU rRNA gene sequences, which are prone to misclassification. We identified 6121 sequences, 625 of which were obtained from cultures, 3053 from cell isolations or enrichments and 2419 from environmental samples. We have corrected the classification for the majority of these curated sequences. The resulting publicly available databases will provide phylogenetically based standards for the improved identification of excavates in ecological and microbiome studies, as well as resources to classify new discoveries in excavate diversity.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10603 - Genetics and heredity (medical genetics to be 3)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Database - The Journal of Biological Databases and Curation

  • ISSN

    1758-0463

  • e-ISSN

  • Volume of the periodical

    neuveden

  • Issue of the periodical within the volume

    NOV 20 2020

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    11

  • Pages from-to

  • UT code for WoS article

    000597225700001

  • EID of the result in the Scopus database

    2-s2.0-85096814458