A freshwater radiation of diplonemids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901518" target="_blank" >RIV/60076658:12310/20:43901518 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/20:00539257
Result on the web
<a href="https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.15209" target="_blank" >https://sfamjournals.onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.15209</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1462-2920.15209" target="_blank" >10.1111/1462-2920.15209</a>
Alternative languages
Result language
angličtina
Original language name
A freshwater radiation of diplonemids
Original language description
Diplonemids are considered marine protists and have been reported among the most abundant and diverse eukaryotes in the world oceans. Recently we detected the presence of freshwater diplonemids in Japanese deep freshwater lakes. However, their distribution and abundances in freshwater ecosystems remain unknown. We assessed abundance and diversity of diplonemids from several geographically distant deep freshwater lakes of the world by amplicon-sequencing, shotgun metagenomics and catalysed reporter deposition-fluorescent in situ hybridization (CARD-FISH). We found diplonemids in all the studied lakes, albeit with low abundances and diversity. We assembled long 18S rRNA sequences from freshwater diplonemids and showed that they form a new lineage distinct from the diverse marine clades. Freshwater diplonemids are a sister-group to a marine clade, which are mainly isolates from coastal and bay areas, suggesting a recent habitat transition from marine to freshwater habitats. Images of CARD-FISH targeted freshwater diplonemids suggest they feed on bacteria. Our analyses of 18S rRNA sequences retrieved from single-cell genomes of marine diplonemids show they encode multiple rRNA copies that may be very divergent from each other, suggesting that marine diplonemid abundance and diversity both have been overestimated. These results have wider implications on assessing eukaryotic abundances in natural habitats by using amplicon-sequencing alone. © 2020 Society for Applied Microbiology and John Wiley & Sons Ltd.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Microbiology
ISSN
1462-2912
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
11
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
4658-4668
UT code for WoS article
000572150500001
EID of the result in the Scopus database
2-s2.0-85091365808