Utilization of light energy in phototrophic Gemmatimonadetes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F20%3A43901711" target="_blank" >RIV/60076658:12310/20:43901711 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/20:00536814 RIV/61388971:_____/20:00536814

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S1011134420305352?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1011134420305352?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jphotobiol.2020.112085" target="_blank" >10.1016/j.jphotobiol.2020.112085</a>

Result language

angličtina

Original language name

Utilization of light energy in phototrophic Gemmatimonadetes

Original language description

Gemmatimonas phototrophica is, so far, the only described phototrophic species of the bacterial phylum Gemmatimonadetes. Its cells contain a unique type of photosynthetic complex with the reaction center surrounded by a double ring antenna, however they can also grow in the dark using organic carbon substrates. Its photosynthesis genes were received via horizontal gene transfer from Proteobacteria. This raises two questions; how the horizontally transferred photosynthesis apparatus has integrated into the cellular machinery, and how much light-derived energy actually contributes to the cellular metabolism? To address these points, the photosynthetic reactions were studied on several levels, from photophysics of the reaction center to cellular growth. Flash photolysis measurements and bacteriochlorophyll fluorescence kinetic measurements documented the presence of fully functional type-2 reaction centers with a large light harvesting antenna. When illuminated, the bacterial cells reduced their respiration rate by 58 +/- 5%, revealing that oxidative phosphorylation was replaced by photophosphorylation. Moreover, illumination also more than doubled the assimilation rates of glucose, a sugar that is mostly used for respiration. Finally, light increased the growth rates of Gemmatimonas phototrophica colonies on agar plates. All the presented data provide evidence that photosynthetic complexes are fully integrated into cellular metabolism of Gemmatimonas phototrophica, and are able to provide a substantial amount of energy for its metabolism and growth.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Photochemistry and Photobiology B-Biology

ISSN

1011-1344

e-ISSN

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Volume of the periodical

213

Issue of the periodical within the volume

DEC 2020

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

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UT code for WoS article

000601340800001

EID of the result in the Scopus database

2-s2.0-85096717885