The tetrameric structure of the novel haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903272" target="_blank" >RIV/60076658:12310/21:43903272 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/21:00119187 RIV/00159816:_____/21:00075110 RIV/68407700:21340/21:00351032
Result on the web
<a href="https://scripts.iucr.org/cgi-bin/paper?S2059798321000486" target="_blank" >https://scripts.iucr.org/cgi-bin/paper?S2059798321000486</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1107/S2059798321000486" target="_blank" >10.1107/S2059798321000486</a>
Alternative languages
Result language
angličtina
Original language name
The tetrameric structure of the novel haloalkane dehalogenase DpaA from Paraglaciecola agarilytica NO2
Original language description
Haloalkane dehalogenases (EC 3.8.1.5) are microbial enzymes that catalyse the hydrolytic conversion of halogenated compounds, resulting in a halide ion, a proton and an alcohol. These enzymes are used in industrial biocatalysis, bioremediation and biosensing of environmental pollutants or for molecular tagging in cell biology. The novel haloalkane dehalogenase DpaA described here was isolated from the psychrophilic and halophilic bacterium Paraglaciecola agarilytica NO2, which was found in marine sediment collected from the East Sea near Korea. Gel-filtration experiments and size-exclusion chromatography provided information about the dimeric composition of the enzyme in solution. The DpaA enzyme was crystallized using the sitting-drop vapour-diffusion method, yielding rod-like crystals that diffracted X-rays to 2.0 angstrom resolution. Diffraction data analysis revealed a case of merohedral twinning, and subsequent structure modelling and refinement resulted in a tetrameric model of DpaA, highlighting an uncommon multimeric nature for a protein belonging to haloalkane dehalogenase subfamily I.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Acta crystallographica. Section D - Structural Biology
ISSN
2059-7983
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
MAR 1 2021
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
347-356
UT code for WoS article
000625172500009
EID of the result in the Scopus database
2-s2.0-85102070813