Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903293" target="_blank" >RIV/60076658:12310/21:43903293 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/21:00549673

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00374-021-01587-9" target="_blank" >https://link.springer.com/article/10.1007%2Fs00374-021-01587-9</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00374-021-01587-9" target="_blank" >10.1007/s00374-021-01587-9</a>

Result language

angličtina

Original language name

Biochemical inhibition of acid phosphatase activity in two mountain spruce forest soils

Original language description

The product inhibition of the 4-methylumbelliferyl phosphate (MUB-P) decay rate, a measure of potential acid phosphatase activity, has not been considered in most of the published kinetic studies. The aim of this study was to determine the type and strength of the product inhibition in order to better define reaction conditions at which the acid phosphatase activity assay produces unbiased results. The MUB-P decay rate was measured in the forest floor and topsoil organic horizons of two spruce forest catchments at different initial MUB-P and P-PO43- concentrations. The type and strength of the inhibition was analyzed by non-linear regression. We found that MUB-P decay was competitively inhibited by the P-PO43- but also by dissolved organic P. By using estimated kinetic parameters, we calculated an underestimation of potential acid phosphatase activity by up to similar to 20% at concentrations of added MUB-P as high as 300 mu mol g(-1) dry weight and an incubation time one hour due to background inorganic and organic P concentrations in range of units of mu mol per gram of dry soil. We discuss approaches that can be used to minimize the underestimation and show that the previously defined recommendations might not be always sufficient to avoid the bias. Therefore, we recommend to analyze progress curves at a wide range of initial MUB-P concentrations using non-linear methods when the enzyme assay is optimized for a given soil. When changes in inorganic and/or organic P concentrations are expected, we further recommend measuring background inorganic and organic P concentrations.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10606 - Microbiology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biology and Fertility of Soils

ISSN

0178-2762

e-ISSN

—

Volume of the periodical

57

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

15

Pages from-to

991-1005

UT code for WoS article

000687940100001

EID of the result in the Scopus database

2-s2.0-85113292047