Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F21%3A43903474" target="_blank" >RIV/60076658:12310/21:43903474 - isvavai.cz</a>

Alternative codes found

RIV/60077344:_____/21:00553138

Result on the web

<a href="https://academic.oup.com/nar/article/49/22/12986/6457957" target="_blank" >https://academic.oup.com/nar/article/49/22/12986/6457957</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkab1204" target="_blank" >10.1093/nar/gkab1204</a>

Result language

angličtina

Original language name

Dynamic queuosine changes in tRNA couple nutrient levels to codon choice in Trypanosoma brucei

Original language description

Every type of nucleic acid in cells undergoes programmed chemical post-transcriptional modification. Generally, modification enzymes use substrates derived from intracellular metabolism, one exception is queuine (q)/queuosine (Q), which eukaryotes obtain from their environment; made by bacteria and ultimately taken into eukaryotic cells via currently unknown transport systems. Here, we use a combination of molecular, cell biology and biophysical approaches to show that in Trypanosoma brucei tRNA Q levels change dynamically in response to concentration variations of a sub-set of amino acids in the growth media. Most significant were variations in tyrosine, which at low levels lead to increased Q content for all the natural tRNAs substrates of tRNA-guanine transglycosylase (TGT). Such increase results from longer nuclear dwell time aided by retrograde transport following cytoplasmic splicing. In turn high tyrosine levels lead to rapid decrease in Q content. Importantly, the dynamic changes in Q content of tRNAs have negligible effects on global translation or growth rate but, at least, in the case of tRNA(Tyr) it affected codon choice. These observations have implications for the occurrence of other tunable modifications important for &apos;normal&apos; growth, while connecting the intracellular localization of modification enzymes, metabolites and tRNAs to codon selection and implicitly translational output.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

—

Volume of the periodical

49

Issue of the periodical within the volume

22

Country of publishing house

GB - UNITED KINGDOM

Number of pages

14

Pages from-to

12986-12999

UT code for WoS article

000736046000032

EID of the result in the Scopus database

2-s2.0-85123328349