Aging of common carp (Cyprinus carpio L.) sperm induced by short-term storage does not alter global DNA methylation and specific histone modifications in offspring

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F23%3A43906274" target="_blank" >RIV/60076658:12310/23:43906274 - isvavai.cz</a>

Alternative codes found

RIV/60076658:12520/23:43906274

Result on the web

<a href="https://doi.org/10.1016/j.aquaculture.2023.739484" target="_blank" >https://doi.org/10.1016/j.aquaculture.2023.739484</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Aging of common carp (Cyprinus carpio L.) sperm induced by short-term storage does not alter global DNA methylation and specific histone modifications in offspring

Original language description

Short-term storage of sperm is a useful tool to synchronize the availability of male and female gametes for fertilization in a hatchery. Induced aging by short-term storage has been shown to affect fish spermatozoa phenotypes including morphology and motility kinetics. However, its effects on epigenetics of sperm and the resulting embryos have not been investigated. In the present study, sperm of common carp (Cyprinus carpio) was stored in vivo and/or in vitro using an extender, and spermatozoa motility kinetics, viability, and epigenetics were analyzed. We observed that spermatozoa motility and velocity decreased following in vivo and/or in vitro storage from 3 to 6.5 days, but spermatozoa viability remained stable. At the same time, global DNA methylation, DNA hydroxymethylation, and specific histone acetylation and methylation were not affected by sperm aging. Further analysis showed no variation in global DNA methylation during embryogenesis when short-term stored sperm in the extender was used for fertilization compared with embryos produced from fresh sperm. The results suggest the reliability of short-term storage of sperm using an extender in fertilization and the production of the next generation of embryos. However, larval growth and performance need to be elucidated for these fish. This study has shed light on the potential application of extenders in storage of sperm in common carp breeding programs to manage selection, genomic manipulation and genetic resources conservation

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

40103 - Fishery

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Aquaculture

ISSN

0044-8486

e-ISSN

1873-5622

Volume of the periodical

571

Issue of the periodical within the volume

neuvedeno

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

14

Pages from-to

739484

UT code for WoS article

001053326600001

EID of the result in the Scopus database

2-s2.0-85150857005