The effect of preservation on biomass and length estimates and its variation within and between two mayfly species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60076658%3A12310%2F23%3A43906461" target="_blank" >RIV/60076658:12310/23:43906461 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s10201-023-00715-8" target="_blank" >https://link.springer.com/article/10.1007/s10201-023-00715-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10201-023-00715-8" target="_blank" >10.1007/s10201-023-00715-8</a>

Result language

angličtina

Original language name

The effect of preservation on biomass and length estimates and its variation within and between two mayfly species

Original language description

Biomass estimation offers insight into aquatic ecosystem processes and represents a powerful tool for measuring biomass in communities. However, the accuracy of this estimate depends considerably on the sample preservation method and biological traits of species. We quantified the effect of commonly used preservatives (70% ethanol, 4% formaldehyde, freezing), preservation time and initial body length on the estimation bias of body length, wet mass and dry mass in two mayfly species with dorsoventrally flattened (Rhithrogena carpatoalpina) and cylindrically elongated (Habroleptoides confusa) bodies. Freezing caused the largest relative bias in the length estimates (up to 18%). Both ethanol and freezing led to significant underestimation of wet mass of preserved animals (up to 26%). The most severe biases were observed in dry weights, where all preservation methods reduced body mass by up to 89%. Importantly, initial body mass influenced the bias caused by preservation, and the larger animals were less affected by mass losses. In contrast, larger individuals were subject to greater length reduction. Additionally, the length-mass relationship for R. carpatoalpina, based on fresh mass of living animals, was significantly different from relationships based on preserved animals. Overall, formaldehyde caused the least biased estimates of length and biomass in both examined species. This case study indicates that direct measurements of unpreserved individuals or length-mass equations based on fresh material are likely the best ways to avoid severe underestimation of biomass in aquatic insects. However, more studies covering a broader range of taxa are needed to reach general conclusions.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10617 - Marine biology, freshwater biology, limnology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Limnology

ISSN

1439-8621

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

3

Country of publishing house

JP - JAPAN

Number of pages

11

Pages from-to

181-191

UT code for WoS article

000963026600001

EID of the result in the Scopus database

2-s2.0-85151572608